ArticleBiophysical journal1997
Single-channel properties of ionic channels gated by cyclic nucleotides.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- Gating intermediates reveal inhibitory role of the voltage sensor in a cyclic nucleotide-modulated ion channel.Nature communications · 2022Article
- CNG channel structure, function, and gating: a tale of conformational flexibility.Pflugers Archiv : European journal of physiology · 2021Review
- Proton transfer unlocks inactivation in cyclic nucleotide-gated A1 channels.The Journal of physiology · 2015Article
- A ring of threonines in the inner vestibule of the pore of CNGA1 channels constitutes a binding site for permeating ions.The Journal of physiology · 2012Article
- Speed, sensitivity, and stability of the light response in rod and cone photoreceptors: facts and models.Progress in retinal and eye research · 2012Review
- Cyclic nucleotide-gated channel block by hydrolysis-resistant tetracaine derivatives.Journal of medicinal chemistry · 2011Article
- Gating in CNGA1 channels.Pflugers Archiv : European journal of physiology · 2010Review
- Intrinsic versus extrinsic voltage sensitivity of blocker interaction with an ion channel pore.The Journal of general physiology · 2010Article
- Mutations reveal voltage gating of CNGA1 channels in saturating cGMP.The Journal of general physiology · 2009Article
- State-dependent accessibility of the P-S6 linker of pacemaker (HCN) channels supports a dynamic pore-to-gate coupling model.The Journal of membrane biology · 2009Article
- Thermodynamics of activation gating in olfactory-type cyclic nucleotide-gated (CNGA2) channels.Biophysical journal · 2008Article
- Bidirectional temperature-sensing by a single thermosensory neuron in C. elegans.Nature neuroscience · 2008Article
- A comparison of electrophysiological properties of the CNGA1, CNGA1tandem and CNGA1cys-free channels.European biophysics journal : EBJ · 2008Article
- Gating of cyclic nucleotide-gated (CNGA1) channels by cGMP jumps and depolarizing voltage steps.Biophysical journal · 2006Article
- Effects of permeating ions and cGMP on gating and conductance of rod-type cyclic nucleotide-gated (CNGA1) channels.The Journal of physiology · 2004Article
- Molecular basis of an inherited form of incomplete achromatopsia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- A cysteine scan of the inner vestibule of cyclic nucleotide-gated channels reveals architecture and rearrangement of the pore.The Journal of general physiology · 2003Article
- Dequalinium: a novel, high-affinity blocker of CNGA1 channels.The Journal of general physiology · 2003Article
- Movement of the C-helix during the gating of cyclic nucleotide-gated channels.Biophysical journal · 2002Article
- Evidence for non-independent gating of P2X2 receptors expressed in Xenopus oocytes.BMC neuroscience · 2002Article
Corrections and comments
- Commented on by
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper presents an extensive analysis of single-channel properties of cyclic nucleotide gated (CNG) channels, obtained by injecting into Xenopus laevis oocytes the mRNA encoding for the alpha and beta subunits from bovine rods. When the alpha and beta subunits of the CNG channel are coexpressed, at least three types of channels with different properties are observed. One type of channel has well-resolved, multiple conductive levels at negative voltages, but not at positive voltages. The other two types of channel are characterized by flickering openings, but are distinguished because they have a low and a high conductance. The alpha subunit of CNG channels has a well-defined conductance of about 28 pS, but multiple conductive levels are observed in mutant channels E363D and T364M. The conductance of these open states is modulated by protons and the membrane voltage, and has an activation energy around 44 kJ/mol. The relative probability of occupying any of these open states is independent of the cGMP concentration, but depends on extracellular protons. The open probability in the presence of saturating cGMP was 0.78, 0.47, 0.5, and 0.007 in the w.t. and mutants E363D, T364M, and E363G, and its dependence on temperature indicates that the thermodynamics of the transition between the closed and open state is also affected by mutations in the pore region. These results suggest that CNG channels have different conductive levels, leading to the existence of multiple open states in homomeric channels and to the flickering behavior in heteromeric channels, and that the pore is an essential part of the gating of CNG channels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.