ArticleThe American journal of pathology1997
Sensitive in situ hybridization with catalyzed reporter deposition, streptavidin-Nanogold, and silver acetate autometallography: detection of single-copy human papillomavirus.
Article in The American journal of pathology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 105 citations in OpenAlex.
- Emerging Trends of Gold Nanostructures for Point-of-Care Biosensor-Based Detection of COVID-19.Molecular biotechnology · 2025Review
- Recent Advances in HPV Detection: From Traditional Methods to Nanotechnology and the Application of Quantum Dots.International journal of nanomedicine · 2025Review
- Research Progress of Polysaccharide-Gold Nanocomplexes in Drug Delivery.Pharmaceutics · 2024Review
- Nanostructures for prevention, diagnosis, and treatment of viral respiratory infections: from influenza virus to SARS-CoV-2 variants.Journal of nanobiotechnology · 2023Review
- Recent advances in carbon quantum dots for virus detection, as well as inhibition and treatment of viral infection.Nano convergence · 2022Review
- Advances in Nanotechnology as a Potential Alternative for Plant Viral Disease Management.Frontiers in microbiology · 2022Review
- Nanotechnology based solutions to combat zoonotic viruses with special attention to SARS, MERS, and COVID 19: Detection, protection and medication.Microbial pathogenesis · 2021Review
- Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA.BMC biotechnology · 2021Article
- Rapid diagnostics of coronavirus disease 2019 in early stages using nanobiosensors: Challenges and opportunities.Talanta · 2021Review
- Nanotechnology-Based Weapons to Combat Human Papillomavirus Infection Associated Diseases.Frontiers in chemistry · 2021Review
- Nanomaterials and Nanotechnology-Associated Innovations against Viral Infections with a Focus on Coronaviruses.Nanomaterials (Basel, Switzerland) · 2020Review
- Applications of gold nanoparticles in virus detection.Theranostics · 2018Review
- Single-molecule protein arrays enabled by scanning probe block copolymer lithography.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Human papillomavirus (HPV) detection using in situ hybridization in histologic samples: correlations with cytologic changes and polymerase chain reaction HPV detection.American journal of clinical pathology · 2011Article
- From lateral flow devices to a novel nano-color microfluidic assay.Sensors (Basel, Switzerland) · 2009Article
- Genotyping of phenotypically defined cells in neoplasia: enhanced immunoFISH via tyramide signal amplification (TSA) segregates immunophenotypically-defined cell populations for gated genotyping.Journal of molecular histology · 2007Article
- Novel bright field molecular morphology methods for detection of HER2 gene amplification.Journal of molecular histology · 2004Article
- In situ localisation of Yersinia enterocolitica by catalysed reported deposition signal amplification.Journal of clinical pathology · 1998Article
- Fixation conditions for DNA and RNA in situ hybridization: a reassessment of molecular morphology dogma.The American journal of pathology · 1998Article
- The efficacy of in situ PCR, CARD and nanogold systems for gene detection.The American journal of pathology · 1997Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The usefulness of standard in situ hybridization for viral nucleic acid detection is occasionally limited by its sensitivity limit of 10 to 50 copies per cell. A modified version of the recently described signal amplification method, catalyzed reporter deposition (CARD), and its application to formalin-fixed cells and tissue sections is presented. Deposition of the reporter is facilitated by using horseradish peroxidase catalyzing the deposition of biotinylated tyramide on the location of the probe target. The biotin accumulation created is usually detected with streptavidin-labeled enzymes or fluorochromes. In the present investigation, this step was replaced by streptavidin-Nanogold and combined with silver acetate autometallography. This resulted in deep-black precipitation at positive in situ hybridized reaction sites. The sensitivity of this new approach was tested with a biotinylated, genomic probe specific for human papillomavirus (HPV)-16/18. SiHa cells, a cervical carcinoma-derived cell line with one to two HPV16 copies per cell, and 10 histologically confirmed cervical carcinomas were used for the study. All samples were previously HPV16 positive with solution polymerase chain reaction, but only two of the cervical carcinomas were positive with standard in situ hybridization with barely visible signals. When employing CARD-Nanogold, SiHa cells and 9 of 10 biopsies proved positive with marked signals. It is concluded that this nonisotopic method can detect single viral copies in situ in routinely fixed material and may have the potential to replace in situ polymerase chain reaction in many applications.
Indexed as
Identifiers
9137082PMC1858216W2133138424What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.