ArticleGenetics1997
FAR1, a negative regulatory locus required for the repression of the nitrate reductase gene in Chlamydomonas reinhardtii.
Article in Genetics, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 38 citations in OpenAlex.
- Identification of a gene controlling levels of the copper response regulator 1 transcription factor in Chlamydomonas reinhardtii.The Plant cell · 2024Article
- A global analysis of IFT-A function reveals specialization for transport of membrane-associated proteins into cilia.Journal of cell science · 2019Article
- Nickel and low CO₂-controlled motility in Chlamydomonas through complementation of a paralyzed flagella mutant with chemically regulated promoters.BMC plant biology · 2011Article
- A soluble guanylate cyclase mediates negative signaling by ammonium on expression of nitrate reductase in Chlamydomonas.The Plant cell · 2010Article
- Nitrate assimilation in Chlamydomonas.Eukaryotic cell · 2008Review
- The NIT1 promoter allows inducible and reversible silencing of centrin in Chlamydomonas reinhardtii.Eukaryotic cell · 2005Article
- Functional genomics of the regulation of the nitrate assimilation pathway in Chlamydomonas.Plant physiology · 2005Article
- Paths toward algal genomics.Plant physiology · 2005Review
- A genome's-eye view of the light-harvesting polypeptides of Chlamydomonas reinhardtii.Current genetics · 2004Review
- Chlamydomonas reinhardtii at the crossroads of genomics.Eukaryotic cell · 2003Review
- Identification of short promoter regions involved in the transcriptional expression of the nitrate reductase gene in Chlamydomonas reinhardtii.Plant molecular biology · 2001Article
- Transcriptional regulation of the Nia1 gene encoding nitrate reductase in Chlamydomonas reinhardtii: effects of various environmental factors on the expression of a reporter gene under the control of the Nia1 promoter.Plant molecular biology · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In Chlamydomonas reinhardtii, the genes required for nitrate assimilation, including the gene encoding nitrate reductase (NIT1), are subject to repression by ammonia. To study the mechanism of ammonia repression, we employed two approaches to search for mutants with defective repression of NIT1 gene expression. (1) PF14, a gene required for flagellar function, was used as a reporter gene for expression from the NIT1 promoter. When introduced into a pf14 mutant host, the NIT1;PF14 chimeric construct produced a transformant (T10-10B) with a conditional swimming phenotype. Spontaneous mutants with defective ammonia repression of the NIT1 promoter were screened for by isolating cells that gained constitutive motility. (2) Insertional mutagenesis was performed, followed by screening for chlorate sensitivity in the presence of ammonia ion. One insertional mutant and six spontaneous mutants were allelic and defined a new gene, FAR1 (free from ammonia repression). FAR1 was mapped to Linkage Group I, 7.7 cM to the right of the centromere. The far1-1 mutant strain was used to clone DNA adjacent to the site of plasmid insertion, which was then used as a hybridization probe to clone the FAR1 gene from wild type.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.