ArticleThe EMBO journal1997
Dynamic relocation of transcription and splicing factors dependent upon transcriptional activity.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
85 citing papers in PubMed, 194 citations in OpenAlex.
- Non-random inter-speckle material transfer mediated by dynamic multiphase interchromatin connections.The EMBO journal · 2026Article
- Review
- Does co-transcriptional regulation of alternative splicing mediate plant stress responses?Nucleic acids research · 2019Review
- Epstein-Barr Virus-Induced Nodules on Viral Replication Compartments Contain RNA Processing Proteins and a Viral Long Noncoding RNA.Journal of virology · 2018Article
- Splicing regulation by long noncoding RNAs.Nucleic acids research · 2018Review
- The long noncoding RNAs NEAT1 and MALAT1 bind active chromatin sites.Molecular cell · 2014Article
- Transcriptional properties of mammalian elongin A and its role in stress response.The Journal of biological chemistry · 2013Article
- Cellular ontogeny of RBMY during human spermatogenesis and its role in sperm motility.Journal of biosciences · 2013Article
- An immunocytochemical study of interchromatin granule clusters in early mouse embryos.BioMed research international · 2013Article
- Nucleosome structural changes induced by binding of non-histone chromosomal proteins HMGN1 and HMGN2.FEBS open bio · 2013Article
- The hnRNP A1 homolog Hrp36 is essential for normal development, female fecundity, omega speckle formation and stress tolerance in Drosophila melanogaster.Journal of biosciences · 2012Article
- Article
- Disruption of nuclear organization during the initial phase of African swine fever virus infection.Journal of virology · 2011Article
- The CDK9/cyclin T1 subunits of P-TEFb in mouse oocytes and preimplantation embryos: a possible role in embryonic genome activation.BMC developmental biology · 2011Article
- Herpesviral replication compartments move and coalesce at nuclear speckles to enhance export of viral late mRNA.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Nuclear speckles.Cold Spring Harbor perspectives in biology · 2011Review
- Spatial Organization and Dynamics of Transcription Elongation and Pre-mRNA Processing in Live Cells.Genetics research international · 2011Article
- Association of adipogenic genes with SC-35 domains during porcine adipogenesis.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Subnuclear targeting of the RNA-binding motif protein RBM6 to splicing speckles and nascent transcripts.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Genotoxic stress causes the accumulation of the splicing regulator Sam68 in nuclear foci of transcriptionally active chromatin.Nucleic acids research · 2010Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Recent interest in understanding the spatial organization of gene expression has focused attention on nuclear structures known as speckles or interchromatin granule clusters (IGCs) revealed by immunofluorescence or electron microscopy. Staining of nuclear factors involved in pre-mRNA splicing or, more recently, transcription, reveals 20-40 speckles per nucleus, resulting in the intriguing suggestion that speckles are nuclear sites of transcription and processing. In contrast, other investigations have observed transcription in other areas of the nucleus. In this study, we have examined the localization of active transcription as detected by uridine incorporation and recently developed RNA polymerase II antibodies, and compared this pattern with that of known splicing and polyadenylation factors. Our results indicate that in actively transcribing cells, transcription and splicing factors are dispersed throughout the nucleus with abundant sites of preferred localization. In contrast, in poorly transcribing cells, polymerase II and splicing factors localize to speckles. In nuclei inactivated for transcription by drugs or heat shock, the speckle type of co-localization is accentuated. These observations suggest that bulk transcription and splicing occur throughout the nucleus during periods of active transcription; and that factors involved in these two processes re-locate to minimal speckle domains during periods of inactive transcription.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.