ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Bitter taste transduction of denatonium in the mudpuppy Necturus maculosus.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Evaluation of Bronchial Receptors with Concentration-Response Curves, Bitterness Assessment, and Computer Simulations.Cell biochemistry and biophysics · 2026Article
- Taste Receptors beyond Taste Buds.International journal of molecular sciences · 2022Review
- Sweet Taste Is Complex: Signaling Cascades and Circuits Involved in Sweet Sensation.Frontiers in human neuroscience · 2021Review
- Structure-Function Analyses of Human Bitter Taste Receptors-Where Do We Stand?Molecules (Basel, Switzerland) · 2020Review
- Sensing Senses: Optical Biosensors to Study Gustation.Sensors (Basel, Switzerland) · 2020Review
- Denatonium inhibits growth and induces apoptosis of airway epithelial cells through mitochondrial signaling pathways.Respiratory research · 2015Article
- Article
- Increases in intracellular calcium via activation of potentially multiple phospholipase C isozymes in mouse olfactory neurons.Frontiers in cellular neuroscience · 2014Article
- Article
- Cholinergic microvillous cells in the mouse main olfactory epithelium and effect of acetylcholine on olfactory sensory neurons and supporting cells.Journal of neurophysiology · 2011Article
- Ryanodine receptors selectively contribute to the formation of taste-evoked calcium signals in mouse taste cells.The European journal of neuroscience · 2010Article
- Calcium signaling in taste cells: regulation required.Chemical senses · 2010Review
- Chemoreception regulates chemical access to mouse vomeronasal organ: role of solitary chemosensory cells.PloS one · 2010Article
- Sodium/calcium exchangers selectively regulate calcium signaling in mouse taste receptor cells.Journal of neurophysiology · 2010Article
- Mitochondrial calcium buffering contributes to the maintenance of Basal calcium levels in mouse taste cells.Journal of neurophysiology · 2008Article
- The transduction channel TRPM5 is gated by intracellular calcium in taste cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Expression of P2Y1 receptors in rat taste buds.Histochemistry and cell biology · 2004Article
- Intracellular Ca2+ and the phospholipid PIP2 regulate the taste transduction ion channel TRPM5.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Individual mouse taste cells respond to multiple chemical stimuli.The Journal of physiology · 2002Article
- Taste receptor cells that discriminate between bitter stimuli.Science (New York, N.Y.) · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
Bitter substances are a structurally diverse group of compounds that appear to act via several transduction mechanisms. The bitter-tasting denatonium ion has been proposed to act via two different G-protein-regulated pathways, one involving inositol 1,4, 5-trisphosphate and raised intracellular calcium levels, the other involving phosphodiesterase and membrane depolarization via a cyclic nucleotide-suppressible cation channel. The aim of the present study was to examine these transduction mechanisms in taste cells of the mudpuppy Necturus maculosus by calcium-imaging and whole-cell recording. Denatonium benzoate increased intracellular calcium levels and induced an outward current independently of extracellular calcium. The denatonium-induced increase in intracellular calcium was inhibited by U73122, an inhibitor of phospholipase C, and by thapsigargin, an inhibitor of calcium transport into intracellular stores. The denatonium-induced outward current was blocked by GDP-beta-S, a blocker of G-protein activation. Neither resting nor denatonium-induced intracellular calcium levels were affected by inhibition of phosphodiesterase (with IBMX) or adenylate cyclase (with SQ22536) or by raising intracellular cyclic nucleotides directly (with cell permeant analogs). Our results support the hypothesis that denatonium is transduced via a G-protein cascade involving phospholipase C, inositol 1,4,5-trisphosphate, and raised intracellular calcium levels. Our results do not support the hypothesis that denatonium is transduced via phosphodiesterase and cAMP.
Indexed as
Identifiers
9133381PMC6573680W2089077302What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.