ArticleJournal of bioenergetics and biomembranes1996
The mitochondrial inner membrane anion channel is inhibited by DIDS.
Article in Journal of bioenergetics and biomembranes, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 62 citations in OpenAlex.
- Targeting ferroptosis in cancer: molecular mechanisms and emerging therapeutic strategies.Biochemistry and biophysics reports · 2026Review
- Recent progress in ferroptosis: inducers and inhibitors.Cell death discovery · 2022Review
- Molecular logic of salt taste reception in special reference to transmembrane channel-like 4 (TMC4).The journal of physiological sciences : JPS · 2022Review
- Mitochondrial ion channels in cardiac function.American journal of physiology. Cell physiology · 2021Review
- TMC4 is a novel chloride channel involved in high-concentration salt taste sensation.The journal of physiological sciences : JPS · 2021Article
- Anion Channels of Mitochondria.Handbook of experimental pharmacology · 2017Review
- Electro-pharmacological profiles of two brain mitoplast anion channels: Inferences from single channel recording.EXCLI journal · 2017Article
- Appearance of fast astrocytic component in voltage-sensitive dye imaging of neural activity.Molecular brain · 2015Article
- The mitochondrial translocator protein and arrhythmogenesis in ischemic heart disease.Oxidative medicine and cellular longevity · 2015Review
- Article
- Regulation of mitochondrial function by voltage dependent anion channels in ethanol metabolism and the Warburg effect.Biochimica et biophysica acta · 2012Review
- An in vitro ischemic penumbral mimic perfusate increases NADPH oxidase-mediated superoxide production in cultured hippocampal neurons.Brain research · 2012Article
- Metabolic stress, reactive oxygen species, and arrhythmia.Journal of molecular and cellular cardiology · 2012Review
- Single channel characterization of the mitochondrial ryanodine receptor in heart mitoplasts.The Journal of biological chemistry · 2011Article
- P2X(7) receptor antagonists display agonist-like effects on cell signaling proteins.Biochimica et biophysica acta · 2011Article
- Cardiac mitochondria and arrhythmias.Cardiovascular research · 2010Review
- Nicotine induces resistance to chemotherapy by modulating mitochondrial signaling in lung cancer.American journal of respiratory cell and molecular biology · 2009Article
- Ethanol exposure decreases mitochondrial outer membrane permeability in cultured rat hepatocytes.Archives of biochemistry and biophysics · 2009Article
- Air pollution induces enhanced mitochondrial oxidative stress in cystic fibrosis airway epithelium.FEBS letters · 2008Article
- Hydrogen sulfide decreases adenosine triphosphate levels in aortic rings and leads to vasorelaxation via metabolic inhibition.Life sciences · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The mitochondrial inner membrane anion channel (IMAC) is a channel, identified by flux studies in intact mitochondria, which has a broad anion selectivity and is maintained closed or inactive by matrix Mg2+ and H+. We now present evidence that this channel, like many other chloride/anion channels, is reversibly blocked/inhibited by stilbene-2,2'-disulfonates. Inhibition of malonate transport approaches 100% with IC50 values of 26, 44, and 88 mu M for DIDS, H2-DIDS, and SITS respectively and Hill coefficients < or = 1. In contrast, inhibition of Cl- transport is incomplete, reaching a maximum of about 30% at pH 7.4 and 65% at pH 8.4 with an IC50 which is severalfold higher than that for malonate. The IC50 for malonate transport is decreased about 50% by pretreatment of the mitochondria with N-ethylmaleimide. Raising the assay pH from 7.4 to 8.4 increases the IC50 by about 50%, but under conditions where only the matrix pH is made alkaline the IC50 is decreased slightly. These properties and competition studies suggest that DIDS inhibits by binding to the same site as Cibacron blue 3GA. In contrast, DIDS does not appear to compete with the fluorescein derivative Erythrosin B for inhibition. These findings not only provide further evidence that IMAC may be more closely related to other "Cl-" channels than previously thought, but also suggest that other Cl- channels may be sensitive to some of the many regulators of IMAC which have been identified.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.