ArticleThe EMBO journal1997
The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 122 citations in OpenAlex.
- Article
- Neuronal Pnn Deficiency Increases Oxidative Stress and Exacerbates Cerebral Ischemia/Reperfusion Injury in Mice.Antioxidants (Basel, Switzerland) · 2022Article
- Splicing of long non-coding RNAs primarily depends on polypyrimidine tract and 5' splice-site sequences due to weak interactions with SR proteins.Nucleic acids research · 2019Article
- Exploring the Crosstalk BetweenFrontiers in genetics · 2018Review
- Nuclear bodies reorganize during myogenesis in vitro and are differentially disrupted by expression of FSHD-associated DUX4.Skeletal muscle · 2016Article
- TDP-43 functions within a network of hnRNP proteins to inhibit the production of a truncated human SORT1 receptor.Human molecular genetics · 2016Article
- Diverse regulation of 3' splice site usage.Cellular and molecular life sciences : CMLS · 2015Review
- Role of six single nucleotide polymorphisms, risk factors in coronary disease, in OLR1 alternative splicing.RNA (New York, N.Y.) · 2015Article
- Conserved proline-directed phosphorylation regulates SR protein conformation and splicing function.The Biochemical journal · 2015Article
- An intronic deletion in the PROM1 gene leads to autosomal recessive cone-rod dystrophy.Molecular vision · 2015Article
- Regulation of a novel isoform of Receptor Expression Enhancing Protein REEP6 in rod photoreceptors by bZIP transcription factor NRL.Human molecular genetics · 2014Article
- Isolated pseudo-RNA-recognition motifs of SR proteins can regulate splicing using a noncanonical mode of RNA recognition.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Review
- Genome-wide analysis reveals SR protein cooperation and competition in regulated splicing.Molecular cell · 2013Article
- A novel actin mRNA splice variant regulates ACTG1 expression.PLoS genetics · 2013Article
- Regulation of alternative splicing within the supraspliceosome.Journal of structural biology · 2012Article
- Characterization of tissue-specific and developmentally regulated alternative splicing of exon 64 in the COL5A1 gene.Connective tissue research · 2012Article
- SR proteins induce alternative exon skipping through their activities on the flanking constitutive exons.Molecular and cellular biology · 2011Article
- Vitamin A metabolite, all-trans-retinoic acid, mediates alternative splicing of protein kinase C deltaVIII (PKCdeltaVIII) isoform via splicing factor SC35.The Journal of biological chemistry · 2010Article
- Rapid-response splicing reporter screens identify differential regulators of constitutive and alternative splicing.Molecular and cellular biology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exons 6A and 6B of the chicken beta-tropomyosin gene are mutually exclusive and selected in a tissue-specific manner. Exon 6A is present in non-muscle and smooth muscle cells, while exon 6B is present in skeletal muscle cells. In this study we have investigated the mechanism underlying exon 6A recognition in non-muscle cells. Previous reports have identified a pyrimidine-rich intronic enhancer sequence (S4) downstream of exon 6A as essential for exon 6A 5'-splice site recognition. We show here that preincubation of HeLa cell extracts with an excess of RNA containing this sequence specifically inhibits exon 6A recognition by the splicing machinery. Splicing inhibition by an excess of this RNA can be rescued by addition of the SR protein ASF/SF2, but not by the SR proteins SC35 or 9G8. ASF/SF2 stimulates exon 6A splicing through specific interaction with the enhancer sequence. Surprisingly, SC35 behaves as an inhibitor of exon 6A splicing, since addition to HeLa nuclear extracts of increasing amounts of the SC35 protein completely abolish the stimulatory effect of ASF/SF2 on exon 6A splicing. We conclude that exon 6A recognition in vitro depends on the ratio of the ASF/SF2 to SC35 SR proteins. Taken together our results suggest that variations in the level or activity of these proteins could contribute to the tissue-specific choice of beta-tropomyosin exon 6A. In support of this we show that SR proteins isolated from skeletal muscle tissues are less efficient for exon 6A stimulation than SR proteins isolated from HeLa cells.
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