ArticleThe EMBO journal1997
In vivo commitment to splicing in yeast involves the nucleotide upstream from the branch site conserved sequence and the Mud2 protein.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Autoregulation of RPL7B by inhibition of a structural splicing enhancer.Nucleic acids research · 2025Article
- mRNA nuclear export: how mRNA identity features distinguish functional RNAs from junk transcripts.RNA biology · 2024Review
- Sequence Determinants for Nuclear Retention and Cytoplasmic Export of mRNAs and lncRNAs.Frontiers in genetics · 2018Review
- Nineteen complex-related factor Prp45 is required for the early stages of cotranscriptional spliceosome assembly.RNA (New York, N.Y.) · 2017Article
- Global analysis of pre-mRNA subcellular localization following splicing inhibition by spliceostatin A.RNA (New York, N.Y.) · 2017Article
- SF1 Phosphorylation Enhances Specific Binding to U2AFBiophysical journal · 2016Article
- The Evolutionarily-conserved Polyadenosine RNA Binding Protein, Nab2, Cooperates with Splicing Machinery to Regulate the Fate of pre-mRNA.Molecular and cellular biology · 2016Article
- Identification of new branch points and unconventional introns in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2016Article
- Links between mRNA splicing, mRNA quality control, and intellectual disability.RNA & disease (Houston, Tex.) · 2016Article
- Histone H3K36 methylation regulates pre-mRNA splicing in Saccharomyces cerevisiae.RNA biology · 2016Article
- Rapid identification of mRNA processing defects with a novel single-cell yeast reporter.RNA (New York, N.Y.) · 2014Article
- Cotranscriptional recruitment of yeast TRAMP complex to intronic sequences promotes optimal pre-mRNA splicing.Nucleic acids research · 2014Article
- Splicing fidelity: DEAD/H-box ATPases as molecular clocks.RNA biology · 2013Review
- The pre-mRNA retention and splicing complex controls tRNA maturation by promoting TAN1 expression.Nucleic acids research · 2013Article
- Inhibition of mRNA maturation in trypanosomes causes the formation of novel foci at the nuclear periphery containing cytoplasmic regulators of mRNA fate.Journal of cell science · 2012Article
- Coordinated regulation of neuronal mRNA steady-state levels through developmentally controlled intron retention.Genes & development · 2012Article
- RNA decay and RNA silencing in plants: competition or collaboration?Frontiers in plant science · 2011Article
- The DEAH box ATPases Prp16 and Prp43 cooperate to proofread 5' splice site cleavage during pre-mRNA splicing.Molecular cell · 2010Article
- Spliceosome discards intermediates via the DEAH box ATPase Prp43p.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- An endoribonuclease functionally linked to perinuclear mRNP quality control associates with the nuclear pore complexes.PLoS biology · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pre-mRNA splicing is a stepwise nuclear process involving intron recognition and the assembly of the spliceosome followed by intron excision. We previously developed a pre-mRNA export assay that allows the discrimination between early steps of spliceosome formation and splicing per se. Here we present evidence that these two assays detect different biochemical defects for point mutations. Mutations at the 5' splice site lead to pre-mRNA export, whereas 3' splice site mutations do not. A genetic screen applied to mutants in the branch site region shows that all positions in the conserved TACTAAC sequence are important for intron recognition. An exhaustive analysis of pre-mRNA export and splicing defects of these mutants shows that the in vivo recognition of the branch site region does not involve the base pairing of U2 snRNA with the pre-mRNA. In addition, the nucleotide preceding the conserved TACTAAC sequence contributes to the recognition process. We show that a T residue at this position allows for optimal intron recognition and that in natural introns, this nucleotide is also used preferentially. Moreover, the Mud2 protein is involved in the recognition of this nucleotide, thus establishing a role for this factor in the in vivo splicing pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.