ArticleThe EMBO journal1997
Voltage-dependent modulation of T-type calcium channels by protein tyrosine phosphorylation.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 102 citations in OpenAlex.
- Review
- Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1.International journal of molecular sciences · 2019Article
- Interactions between oestrogen and 1α,25(OH)Basic and clinical andrology · 2017Article
- T-type Ca2+ channels in spermatogenic cells and sperm.Pflugers Archiv : European journal of physiology · 2014Review
- Flow cytometry analysis reveals a decrease in intracellular sodium during sperm capacitation.Journal of cell science · 2012Article
- Neuroprotection by lomerizine, a prophylactic drug for migraine, against hydrogen peroxide-induced hippocampal neurotoxicity.Molecular and cellular biochemistry · 2011Article
- Neuronal T-type calcium channels: what's new? Iftinca: T-type channel regulation.Journal of medicine and life · 2011Review
- Acrosome reaction: relevance of zona pellucida glycoproteins.Asian journal of andrology · 2011Review
- Activation of protein kinase C augments T-type Ca2+ channel activity without changing channel surface density.The Journal of physiology · 2006Article
- Low-voltage-activated ("T-Type") calcium channels in review.Journal of bioenergetics and biomembranes · 2003Review
- Modulation and pharmacology of low voltage-activated ("T-Type") calcium channels.Journal of bioenergetics and biomembranes · 2003Review
- Article
- Speract induces calcium oscillations in the sperm tail.The Journal of cell biology · 2003Article
- Cloning and expression of the human T-type channel Ca(v)3.3: insights into prepulse facilitation.Biophysical journal · 2002Article
- Article
- Ca(2+) entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reaction.Molecular biology of the cell · 2000Article
- Tyrosine phosphorylation regulates rapid endocytosis in adrenal chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Properties of a novel pH-dependent Ca2+ permeation pathway present in male germ cells with possible roles in spermatogenesis and mature sperm function.The Journal of general physiology · 1998Article
- Splice-variant changes of the Ca(V)3.2 T-type calcium channel mediate voltage-dependent facilitation and associate with cardiac hypertrophy and development.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A T-type Ca2+ channel is expressed during differentiation of the male germ lineage in the mouse and is retained in sperm, where is it activated by contact with the the egg's extracellular matrix and controls sperm acrosomal exocytosis. Here, we examine the regulation of this Ca2+ channel in dissociated spermatogenic cells from the mouse using the whole-cell patch-clamp technique. T currents were enhanced, or facilitated, after strong depolarizations or high frequency stimulation. Voltage-dependent facilitation increased the Ca2+ current by an average of 50%. The same facilitation is produced by antagonists of protein tyrosine kinase activity. Conversely, antagonists of tyrosine phosphatase activity block voltage-dependent facilitation of the current. These data are consistent with the presence of a two-state model, in which T channels are maintained in a low (or zero) conductance state by tonic tyrosine phosphorylation and can be activated to a high conductance state by a tyrosine phosphatase activity. The positive and negative modulation of this channel by the tyrosine phosphorylation state provides a plausible mechanism for the control of sperm activity during the early stages of mammalian fertilization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.