ArticleThe EMBO journal1997
Specific role for the PH domain of dynamin-1 in the regulation of rapid endocytosis in adrenal chromaffin cells.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 75 citations in OpenAlex.
- Chromaffin Cells of the Adrenal Medulla: Physiology, Pharmacology, and Disease.Comprehensive Physiology · 2019Article
- The RTK Interactome: Overview and Perspective on RTK Heterointeractions.Chemical reviews · 2019Review
- Syndapin 3 modulates fusion pore expansion in mouse neuroendocrine chromaffin cells.American journal of physiology. Cell physiology · 2014Article
- Plastid division control: the PDV proteins regulate DRP5B dynamin activity.Plant molecular biology · 2013Article
- Membrane-trafficking sorting hubs: cooperation between PI4P and small GTPases at the trans-Golgi network.Trends in cell biology · 2011Review
- Differential curvature sensing and generating activities of dynamin isoforms provide opportunities for tissue-specific regulation.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Dynamin and myosin regulate differential exocytosis from mouse adrenal chromaffin cells.Cellular and molecular neurobiology · 2010Review
- Membrane insertion of the pleckstrin homology domain variable loop 1 is critical for dynamin-catalyzed vesicle scission.Molecular biology of the cell · 2009Article
- Isoform and splice-variant specific functions of dynamin-2 revealed by analysis of conditional knock-out cells.Molecular biology of the cell · 2008Article
- Quantification of PtdIns(3,4,5)P(3) dynamics in EGF-stimulated carcinoma cells: a comparison of PH-domain-mediated methods with immunological methods.The Biochemical journal · 2008Article
- Real-time detection reveals that effectors couple dynamin's GTP-dependent conformational changes to the membrane.The EMBO journal · 2008Article
- Visualization of dynamins.Methods in cell biology · 2008Review
- Conformational changes in dynamin on GTP binding and oligomerization reported by intrinsic and extrinsic fluorescence.The Biochemical journal · 2005Article
- Imaging direct, dynamin-dependent recapture of fusing secretory granules on plasma membrane lawns from PC12 cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Activation of dynamin II by POPC in giant unilamellar vesicles: a two-photon fluorescence microscopy study.Journal of protein chemistry · 2002Article
- Sustained stimulation shifts the mechanism of endocytosis from dynamin-1-dependent rapid endocytosis to clathrin- and dynamin-2-mediated slow endocytosis in chromaffin cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Physiological stimuli evoke two forms of endocytosis in bovine chromaffin cells.The Journal of physiology · 2001Article
- Sustained stimulation of exocytosis triggers continuous membrane retrieval in rat pituitary somatotrophs.The Journal of physiology · 2001Article
- Signal-dependent membrane targeting by pleckstrin homology (PH) domains.The Biochemical journal · 2000Review
- Enhancement of the dense-core vesicle secretory cycle by glucocorticoid differentiation of PC12 cells: characteristics of rapid exocytosis and endocytosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dynamin plays a key role in the scission event common to various types of endocytosis. We demonstrate that the pleckstrin homology (PH) domain of dynamin-1 is critical in the process of rapid endocytosis (RE) in chromaffin cells. Introduction of this isolated PH domain into cells at concentrations as low as 1 microM completely suppressed RE. PH domains from other proteins, including that from the closely related dynamin-2, were ineffective as inhibitors, even at high concentrations. Mutational studies indicated that a pair of isoform-specific amino acids, located in a variable loop between the first two beta-strands, accounted for the differential effect of the two dynamin PH domains. Switching these amino acids in the dynamin-2 PH domain to the equivalent residues in dynamin-1 (SL-->GI) generated a molecule that blocked RE. Thus, the PH domain of dynamin-1 is essential for RE and exhibits a precise molecular selectivity. As chromaffin cells express both dynamin-1 and -2, we speculate that different isoforms of dynamin may regulate distinct endocytotic processes and that the PH domain contributes to this specificity.
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