ArticlePlanta1997
Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells.
Article in Planta, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Tubulin post-translational modifications are conserved during land plant evolution.BMC plant biology · 2026Article
- Δ3-tubulin impairs mitotic spindle morphology and increases nuclear size in pancreatic cancer cells.Medical molecular morphology · 2024Article
- Lysine acetylation of cytoskeletal proteins: Emergence of an actin code.The Journal of cell biology · 2020Review
- Flax tubulin and CesA superfamilies represent attractive and challenging targets for a variety of genome- and base-editing applications.Functional & integrative genomics · 2020Review
- Posttranslational modification of plant microtubules.Plant signaling & behavior · 2019Article
- Hexavalent chromium-induced differential disruption of cortical microtubules in some Fabaceae species is correlated with acetylation of α-tubulin.Protoplasma · 2016Article
- Affinity Purification and Characterization of Functional Tubulin from Cell Suspension Cultures of Arabidopsis and Tobacco.Plant physiology · 2016Article
- Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue ofFrontiers in plant science · 2016Article
- Tubulin perturbation leads to unexpected cell wall modifications and affects stomatal behaviour in Populus.Journal of experimental botany · 2015Article
- Microtubules in plants.The arabidopsis book · 2015Article
- Cadmium affects microtubule organization and post-translational modifications of tubulin in seedlings of soybean (Glycine max L.).Frontiers in plant science · 2015Article
- Nitrosative stress triggers microtubule reorganization in Arabidopsis thaliana.Journal of experimental botany · 2014Article
- MAPs: cellular navigators for microtubule array orientations in Arabidopsis.Plant cell reports · 2014Review
- Tubulin tyrosine nitration regulates microtubule organization in plant cells.Frontiers in plant science · 2013Article
- Bioinformatic search of plant microtubule-and cell cycle related serine-threonine protein kinases.BMC genomics · 2010Article
- Changes in the accumulation of alpha- and beta-tubulin during bud development in Vitis vinifera L.Planta · 2010Article
- Tyrosine phosphorylation of plant tubulin.Planta · 2008Article
- Differential expansion and expression of alpha- and beta-tubulin gene families in Populus.Plant physiology · 2007Article
- Structural and immunocytochemical characterization of the Ginkgo biloba L. sperm motility apparatus.Protoplasma · 2006Article
- Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex.Protoplasma · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Distribution of post-translationally modified tubulins in cells of Nicotiana tabacum L. was analysed using a panel of specific antibodies. Polyglutamylated, tyrosinated, nontyrosinated, acetylated and delta 2-tubulin variants were detected on alpha-tubulin subunits; polyglutamylation was also found on beta-tubulin subunits. Modified tubulins were detected by immunofluorescence microscopy in interphase microtubules, preprophase bands, mitotic spindles as well as in phragmoplasts. They were, however, located differently in the various microtubule structures. The antibodies against tyrosinated, acetylated and polyglutamylated tubulins gave uniform staining along all microtubules, while antibodies against nontyrosinated and delta 2-tubulin provided dot-like staining of interphase microtubules. Additionally, immunoreactivity of antibodies against acetylated and delta 2-tubulins was strong in the pole regions of mitotic spindles. High-resolution isoelectric focusing revealed 22 tubulin charge variants in N. tabacum suspension cells. Immunoblotting with antibodies TU-01 and TU-06 against conserved antigenic determinants of alpha- and beta-tubulin molecules, respectively, revealed that 11 isoforms belonged to the alpha-subunit and 11 isoforms to the beta-subunit. Whereas antibodies against polyglutamylated, tyrosinated and acetylated tubulins reacted with several alpha-tubulin isoforms, antibodies against nontyrosinated and delta 2-tubulin reacted with only one. The combined data demonstrate that plant tubulin is extensively post-translationally modified and that these modifications participate in the generation of plant tubulin polymorphism.
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