ArticleInfection and immunity1997
Characterization of the nucleotide sequence of the groE operon encoding heat shock proteins chaperone-60 and -10 of Francisella tularensis and determination of the T-cell response to the proteins in individuals vaccinated with F. tularensis.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
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- TetR-based gene regulation systems for Francisella tularensis.Applied and environmental microbiology · 2012Article
- Identification and characterization of novel and potent transcription promoters of Francisella tularensis.Applied and environmental microbiology · 2011Article
- Genetic manipulation of francisella tularensis.Frontiers in microbiology · 2010Article
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- TLR4-mediated activation of dendritic cells by the heat shock protein DnaK from Francisella tularensis.Journal of leukocyte biology · 2008Article
- RipA, a cytoplasmic membrane protein conserved among Francisella species, is required for intracellular survival.Infection and immunity · 2008Article
- Global transcriptional response to mammalian temperature provides new insight into Francisella tularensis pathogenesis.BMC microbiology · 2008Article
- Targeted inactivation of francisella tularensis genes by group II introns.Applied and environmental microbiology · 2008Article
- Characterization and application of a glucose-repressible promoter in Francisella tularensis.Applied and environmental microbiology · 2008Article
- In vivo Himar1-based transposon mutagenesis of Francisella tularensis.Applied and environmental microbiology · 2006Article
- Construction and characterization of a highly efficient Francisella shuttle plasmid.Applied and environmental microbiology · 2004Article
- Protection afforded by heat shock protein 60 from Francisella tularensis is due to copurified lipopolysaccharide.Infection and immunity · 2004Article
- An attenuated strain of the facultative intracellular bacterium Francisella tularensis can escape the phagosome of monocytic cells.Infection and immunity · 2003Article
- Survival and growth of Francisella tularensis in Acanthamoeba castellanii.Applied and environmental microbiology · 2003Article
- Identification of a 71-kilodalton surface-associated Hsp70 homologue in Coxiella burnetii.Infection and immunity · 1998Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The groE operon of Francisella tularensis LVS, encoding the heat shock proteins chaperone-10 (Cpn10) and Cpn60, was sequenced and characterized, and the T-cell response of LVS-vaccinated individuals to the two proteins and the third major chaperone, Ft-DnaK, was assayed. The cpn10 and cpn60 genes were amplified by PCR with degenerate oligonucleotides derived from the N-terminal sequence of the two proteins. The sequence analysis revealed the expected two open reading frames, encoding proteins with estimated Mrs of 10,300 and 57,400. The deduced amino acid sequences closely resembled Cpn10 and Cpn60 proteins of other prokaryotes. The genes constituted a bicistronic operon, the cpn10 gene preceding the cpn60 gene. Upstream of the cpn10 gene, an inverted repeat and motifs similar to -35 and -10 sequences of sigma70-dependent but not of sigma32-dependent promoters of Escherichia coli were found. The inverted repeat of the operon resembled so-called hairpin loops identified in other characterized prokaryotic groE operons lacking sigma32-dependent promoters. Primer extension analysis disclosed one and the same transcription start, irrespective of the presence or absence of heat or oxidative stress. After separation of lysates of the F. tularensis LVS organism by two-dimensional gel electrophoresis, DnaK, Cpn60, and Cpn10 were extracted and used as antigens in T-cell tests. When compared to those from nonvaccinated individuals, T cells from individuals previously vaccinated with live F. tularensis LVS showed an increased proliferative response to DnaK and Cpn60 but not to Cpn10. The present data will facilitate further studies of the involvement of the heat shock proteins in protective immunity to tularemia.
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