ArticleProceedings of the National Academy of Sciences of the United States of America1997
Establishment of methylation-sensitive-representational difference analysis and isolation of hypo- and hypermethylated genomic fragments in mouse liver tumors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
53 citing papers in PubMed, 182 citations in OpenAlex.
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- Ras association domain family member 10 suppresses gastric cancer growth by cooperating with GSTP1 to regulate JNK/c-Jun/AP-1 pathway.Oncogene · 2016Article
- DNA methylation in gastric cancer, related to Helicobacter pylori and Epstein-Barr virus.World journal of gastroenterology · 2014Review
- Accumulation of aberrant DNA methylation during colorectal cancer development.World journal of gastroenterology · 2014Review
- Molecular epigenetics in the management of ovarian cancer: are we investigating a rational clinical promise?Frontiers in oncology · 2014Review
- Combined methylation mapping of 5mC and 5hmC during early embryonic stages in bovine.BMC genomics · 2013Article
- Deciphering the epigenetic code: an overview of DNA methylation analysis methods.Antioxidants & redox signaling · 2013Review
- Global DNA methylation levels in white blood cells as a biomarker for hepatocellular carcinoma risk: a nested case-control study.Carcinogenesis · 2012Article
- Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays.BMC molecular biology · 2011Article
- Biomarkers in Barrett's esophagus and esophageal adenocarcinoma: predictors of progression and prognosis.World journal of gastroenterology · 2010Review
- Methods for genome-wide analysis of DNA methylation in intestinal tumors.Mutation research · 2010Review
- Aberrant DNA methylation in contrast with mutations.Cancer science · 2010Review
- DNA methylation in diploid inbred lines of potatoes and its possible role in the regulation of heterosis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Identification of driver and passenger DNA methylation in cancer by epigenomic analysis.Advances in genetics · 2010Review
- DNA methylation protects hematopoietic stem cell multipotency from myeloerythroid restriction.Nature genetics · 2009Article
- Tissue-specific variation in DNA methylation levels along human chromosome 1.Epigenetics & chromatin · 2009Article
- Epigenetic mechanisms in human adenovirus type 12 oncogenesis.Seminars in cancer biology · 2009Review
- DNA methylation patterns in lung carcinomas.Seminars in cancer biology · 2009Review
- The MIRA method for DNA methylation analysis.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Aberrant DNA methylation associated with bipolar disorder identified from discordant monozygotic twins.Molecular psychiatry · 2008Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methylation of CpG sites in the genome, which is generally conserved during cell replication, is considered to play important roles in cell differentiation and carcinogenesis. However, investigations on changes in methylation status have been limited to known genes. To make a genome-wide search for differentially methylated genes, we developed a methylation-sensitive-representational difference analysis (MS-RDA) method. The representation of the genome was prepared using the methylation-sensitive restriction enzyme HpaII, and the mixture ratio of tester and driver DNAs was optimized to detect differences in methylation status of a single copy per diploid mammalian genome. By performing comparative MS-RDA of one hepatocellular carcinoma and of background liver tissue of one mouse treated with a food carcinogen (2-amino-3,4-dimethylimidazo[4,5-f] quinoline), we were able to identify (i) extensive hypomethylation of long interspersed nuclear element repetitive sequences in a number of hepatocellular carcinomas, (ii) reduction of the gene dosage of their mitochondrial DNA, and (iii) a hypermethylated DNA fragment of unknown origin. Furthermore, by adding the clones obtained in the first MS-RDA to the driver DNA [MS-RDA with elimination of excessive clones (MS-RDA-WEEC)], nine DNA fragments that could not be detected at the first MS-RDA were isolated as differentially methylated DNA fragments. MS-RDA, combined with MS-RDA-WEEC, is thus a promising approach to identify DNA fragments differentially methylated in two DNA sources.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.