ArticleProceedings of the National Academy of Sciences of the United States of America1997
Identification of a novel negative retinoic acid responsive element in the promoter of the human matrix Gla protein gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 51 citations in OpenAlex.
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- Retinoids Repress Human Cardiovascular Cell Calcification With Evidence for Distinct Selective Retinoid Modulator Effects.Arteriosclerosis, thrombosis, and vascular biology · 2020Article
- Small-molecule hormones: molecular mechanisms of action.International journal of endocrinology · 2013Article
- Nuclear receptor signaling inhibits HIV-1 replication in macrophages through multiple trans-repression mechanisms.Journal of virology · 2011Article
- Use of serial analysis of gene expression to reveal the specific regulation of gene expression profile in asthmatic rats treated by acupuncture.Journal of biomedical science · 2009Article
- C/EBP-beta modulates transcription of tubulointerstitial nephritis antigen in obstructive uropathy.Journal of the American Society of Nephrology : JASN · 2009Article
- Intra- and interindividual variation in gene expression in human adipose tissue.Pflugers Archiv : European journal of physiology · 2007Article
- Retinoid-dependent restriction of human immunodeficiency virus type 1 replication in monocytes/macrophages.Journal of virology · 2004Article
- FHL2, a novel tissue-specific coactivator of the androgen receptor.The EMBO journal · 2000Article
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
The vitamin K-dependent matrix Gla protein (MGP) is synthesized in a wide variety of tissues such as lung, heart, kidney, cartilage, and bone. Expression of the MGP gene is regulated by various growth factors, steroid hormones, and the vitamin A metabolite retinoic acid (RA). In this report, we present evidence that RA down-regulates MGP gene expression in different rat and human cell lines via endogenous retinoid receptors [RA receptor (RAR) and retinoid X receptor (RXR)]. Repression of the human MGP (hMGP) gene is specifically mediated by ligand-activated RAR and RXR. Deletion analysis led to the identification of a novel negative response element (NRE) within the hMGP promoter. DNA binding studies performed with bacterially expressed RAR/RXR reveal the formation of a specific heterodimer/NRE complex. Furthermore, electrophoretic mobility-shift assays performed with proteins from RA-treated cells show that endogenous RAR/RXR binds to the NRE. We demonstrate that the NRE contains a CCAAT box and that both RAR/RXR and CCAAT-binding proteins such as c/EBP beta recognize this common regulatory sequence in the hMGP promoter. Our results indicate that RA-mediated repression of the hMGP gene is due to binding of liganded RAR/RXR to a novel negative RA response element.
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