ArticleMolecular and cellular biology1997
A targeted mutation at the T-cell receptor alpha/delta locus impairs T-cell development and reveals the presence of the nearby antiapoptosis gene Dad1.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 45 citations in OpenAlex.
- γδ T Cells and dendritic cells in refractory Lyme arthritis.Journal of leukocyte biology · 2015Review
- Complete TCR-α gene locus control region activity in T cells derived in vitro from embryonic stem cells.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Ectopic T cell receptor-α locus control region activity in B cells is suppressed by direct linkage to two flanking genes at once.PloS one · 2010Article
- Regulatory potential and control of Foxp3 expression in newborn CD4+ T cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Conservation of sequence and structure flanking the mouse and human beta-globin loci: the beta-globin genes are embedded within an array of odorant receptor genes.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- A new element within the T-cell receptor alpha locus required for tissue-specific locus control region activity.Molecular and cellular biology · 1999Article
- Dividing the empire: boundary chromatin elements delimit the territory of enhancers.The EMBO journal · 1999Review
- Adjacent DNA elements dominantly restrict the ubiquitous activity of a novel chromatin-opening region to specific tissues.The EMBO journal · 1997Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Locus control regions are cis gene regulatory elements comprised of DNase I-hypersensitive sites. These regions usually do not stimulate transcription outside of a chromosomal context, and therefore their ability to regulate the expression of genes is thought to occur through the modification of chromatin accessibility. A locus control region is located downstream of the T-cell receptor (TCR) alpha/delta locus on mouse chromosome 14. This locus control region is known to drive T-cell-specific TCR alpha transcription in transgenic mice. In this report, we describe a targeted deletion of this locus control region and show that this mutation acts at a critical checkpoint in alphabeta T-cell development, between the TCR-intermediate and TCR-high stages. Our analysis further reveals that the antiapoptosis gene Dad1 is at the 3' end of the TCR alpha/delta locus and that Dad1 is required for embryogenesis. We show that mouse Dad1 has a broader expression pattern than the TCR genes, in terms of both tissue and temporal specificity. Finally, we report that the chromatin between TCR alpha and Dad1 is DNase I hypersensitive in a variety of cell types, thus correlating with Dad1 expression and raising the possibility that Dad1 regulatory sequences reside in this region.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.