ArticleMolecular and cellular biology1997
An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 75 citations in OpenAlex.
- Combinatorial engineering of enzyme and pathway for efficient β-farnesene bioproduction inSynthetic and systems biotechnology · 2026Article
- Unlocking the Synthetic Potential of Yarrowia lipolytica: Innovating Gene Expression Tools.Microbial biotechnology · 2025Review
- Optimizing the CRISPR/Cas9 system for gene editing inEngineering microbiology · 2025Article
- Advances and perspectives in genetic expression and operation for the oleaginous yeastSynthetic and systems biotechnology · 2024Review
- Identification and Construction of Strong Promoters inMicroorganisms · 2023Article
- A review of synthetic biology tools in Yarrowia lipolytica.World journal of microbiology & biotechnology · 2023Review
- Identification of neutral genome integration sites with high expression and high integration efficiency inSynthetic and systems biotechnology · 2023Article
- Identification of genome integration sites for developing a CRISPR-based gene expression toolkit in Yarrowia lipolytica.Microbial biotechnology · 2022Article
- Revisiting the unique structure of autonomously replicating sequences in Yarrowia lipolytica and its role in pathway engineering.Applied microbiology and biotechnology · 2021Article
- Review
- Orc4 spatiotemporally stabilizes centromeric chromatin.Genome research · 2021Article
- An artificial chromosome ylAC enables efficient assembly of multiple genes in Yarrowia lipolytica for biomanufacturing.Communications biology · 2020Article
- Improving the efficiency of homologous recombination by chemical and biological approaches in Yarrowia lipolytica.PloS one · 2018Article
- Review
- Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Rad51-Rad52 mediated maintenance of centromeric chromatin in Candida albicans.PLoS genetics · 2014Article
- An autonomously replicating sequence for use in a wide range of budding yeasts.FEMS yeast research · 2014Article
- Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.Applied and environmental microbiology · 2011Article
- Chromosomal G + C content evolution in yeasts: systematic interspecies differences, and GC-poor troughs at centromeres.Genome biology and evolution · 2010Article
- Identification of bifunctional delta12/omega3 fatty acid desaturases for improving the ratio of omega3 to omega6 fatty acids in microbes and plants.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Two DNA fragments displaying ARS activity on plasmids in the yeast Yarrowia lipolytica have previously been cloned and shown to harbor centromeric sequences (P. Fournier, A. Abbas, M. Chasles, B. Kudla, D. M. Ogrydziak, D. Yaver, J.-W. Xuan, A. Peito, A.-M. Ribet, C. Feynerol, F. He, and C. Gaillardin, Proc. Natl. Acad. Sci. USA 90:4912-4916, 1993; and P. Fournier, L. Guyaneux, M. Chasles, and C. Gaillardin, Yeast 7:25-36, 1991). We have used the integration properties of centromeric sequences to show that all Y. lipolytica ARS elements so far isolated are composed of both a replication origin and a centromere. The sequence and the distance between the origin and centromere do not seem to play a critical role, and many origins can function in association with one given centromere. A centromeric plasmid can therefore be used to clone putative chromosomal origins coming from several genomic locations, which confer the replicative property on the plasmid. The DNA sequences responsible for initiation in plasmids are short (several hundred base pairs) stretches which map close to or at replication initiation sites in the chromosome. Their chromosomal deletion abolishes initiation, but changing their chromosomal environment does not.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.