ArticleMolecular and cellular biology1997
Plant farnesyltransferase can restore yeast Ras signaling and mating.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Fine mapping of a thrips resistance QTL in Capsicum and the role of diterpene glycosides in the underlying mechanism.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Sensing and signaling of oxidative stress in chloroplasts by inactivation of the SAL1 phosphoadenosine phosphatase.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Towards the systematic mapping and engineering of the protein prenylation machinery in Saccharomyces cerevisiae.PloS one · 2015Article
- Differential effects of prenylation and s-acylation on type I and II ROPS membrane interaction and function.Plant physiology · 2011Article
- Functional analysis of Arabidopsis postprenylation CaaX processing enzymes and their function in subcellular protein targeting.Plant physiology · 2008Article
- Farnesylation directs AtIPT3 subcellular localization and modulates cytokinin biosynthesis in Arabidopsis.Plant physiology · 2008Article
- Dual lipid modification of Arabidopsis Ggamma-subunits is required for efficient plasma membrane targeting.Plant physiology · 2007Article
- Protein geranylgeranyltransferase I is involved in specific aspects of abscisic acid and auxin signaling in Arabidopsis.Plant physiology · 2005Article
- Genome organization in Arabidopsis thaliana: a survey for genes involved in isoprenoid and chlorophyll metabolism.Plant molecular biology · 2003Article
- A cell-specific, prenylation-independent mechanism regulates targeting of type II RACs.The Plant cell · 2002Article
- Hypersensitivity of abscisic acid-induced cytosolic calcium increases in the Arabidopsis farnesyltransferase mutant era1-2.The Plant cell · 2002Article
- Efficient prenylation by a plant geranylgeranyltransferase-I requires a functional CaaL box motif and a proximal polybasic domain.Plant physiology · 2001Article
- Functional requirement of plant farnesyltransferase during development in Arabidopsis.The Plant cell · 2000Article
- Prenylation of the floral transcription factor APETALA1 modulates its function.The Plant cell · 2000Article
- Cloning of the Arabidopsis WIGGUM gene identifies a role for farnesylation in meristem development.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- The prenylation status of a novel plant calmodulin directs plasma membrane or nuclear localization of the protein.The EMBO journal · 1999Article
- Protein prenylation in plants: old friends and new targets.Plant molecular biology · 1999Review
- Arachidonic acid alters tomato HMG expression and fruit growth and induces 3-hydroxy-3-methylglutaryl coenzyme A reductase-independent lycopene accumulationPlant physiology · 1999Article
- Prenylcysteine alpha-carboxyl methyltransferase in suspension-cultured tobacco cellsPlant physiology · 1998Article
- AtKuP1: a dual-affinity K+ transporter from Arabidopsis.The Plant cell · 1998Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Farnesyltransferase (FTase) is a heterodimeric enzyme that modifies a group of proteins, including Ras, in mammals and yeasts. Plant FTase alpha and beta subunits were cloned from tomato and expressed in the yeast Saccharomyces cerevisiae to assess their functional conservation in farnesylating Ras and a-factor proteins, which are important for cell growth and mating. The tomato FTase beta subunit (LeFTB) alone was unable to complement the growth defect of ram1 delta mutant yeast strains in which the chromosomal FTase beta subunit gene was deleted, but coexpression of LeFTB with the plant alpha subunit gene (LeFTA) restored normal growth, Ras membrane association, and mating. LeFTB contains a novel 66-amino-acid sequence domain whose deletion reduces the efficiency of tomato FTase to restore normal growth to yeast ram1 delta strains. Coexpression of LeFTA and LeFTB in either yeast or insect cells yielded a functional enzyme that correctly farnesylated CaaX-motif-containing peptides. Despite their low degree of sequence homology, yeast and plant FTases shared similar in vivo and in vitro substrate specificities, demonstrating that this enzymatic modification of proteins with intermediates from the isoprenoid biosynthesis pathway is conserved in evolutionarily divergent eukaryotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.