ArticleMolecular and cellular biology1997
Position-dependent transcriptional regulation of the murine dihydrofolate reductase promoter by the E2F transactivation domain.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- An E2F5-TFDP1-BRG1 Complex Mediates Transcriptional Activation of MYCN in Hepatocytes.Frontiers in cell and developmental biology · 2021Article
- Systematic discovery of novel eukaryotic transcriptional regulators using sequence homology independent prediction.BMC genomics · 2017Article
- Genetic perspective of retinoblastoma: From present to future.Indian journal of ophthalmology · 2016Review
- The Nuclear Matrix Protein, NRP/B, Acts as a Transcriptional Repressor of E2F-mediated Transcriptional Activity.Journal of cancer prevention · 2014Article
- Nuclear Smad7 Overexpressed in Mesenchymal Cells Acts as a Transcriptional Corepressor by Interacting with HDAC-1 and E2F to Regulate Cell Cycle.Biology open · 2012Article
- Transcription factor effector domains.Sub-cellular biochemistry · 2011Article
- Article
- KIF14 and E2F3 mRNA expression in human retinoblastoma and its phenotype association.Molecular vision · 2009Article
- Article
- Rb2/p130 and protein phosphatase 2A: key mediators of ovarian carcinoma cell growth suppression by all-trans retinoic acid.Oncogene · 2006Review
- Conservation and evolution of cis-regulatory systems in ascomycete fungi.PLoS biology · 2004Article
- Article
- Basal transcriptional regulation of human damage-specific DNA-binding protein genes DDB1 and DDB2 by Sp1, E2F, N-myc and NF1 elements.Nucleic acids research · 2003Article
- Two E2F elements regulate the proliferating cell nuclear antigen promoter differently during leaf development.The Plant cell · 2002Article
- PIN1 is an E2F target gene essential for Neu/Ras-induced transformation of mammary epithelial cells.Molecular and cellular biology · 2002Article
- Cell cycle-dependent recruitment of HDAC-1 correlates with deacetylation of histone H4 on an Rb-E2F target promoter.EMBO reports · 2001Article
- Functional antagonism between E2F family members.Genes & development · 2001Article
- c-Myc is a critical target for c/EBPalpha in granulopoiesis.Molecular and cellular biology · 2001Article
- Cooperation of E2F-p130 and Sp1-pRb complexes in repression of the Chinese hamster dhfr gene.Molecular and cellular biology · 2001Article
- Cloning and characterization of the 5'-upstream sequence governing the cell cycle-dependent transcription of mouse DNA polymerase alpha 68 kDa subunit gene.Nucleic acids research · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Activity of the dihydrofolate reductase (dhfr) promoter increases at the G1-S-phase boundary of the cell cycle. Mutations that abolish protein binding to an E2F element in the dhfr promoter also abolish the G1-S-phase increase in dhfr transcription, indicating that transcriptional regulation is mediated by the E2F family of proteins. To investigate the mechanism by which E2F regulates dhfr transcription, we moved the E2F element upstream and downstream of its natural position in the promoter. We found that the E2F element confers growth regulation to the dhfr promoter only when it is proximal to the transcription start site. Using a heterologous E2F element, we showed that position-dependent regulation is a property that is promoter specific, not E2F element specific. We demonstrated that E2F-mediated growth regulation of dhfr transcription requires activation of the dhfr promoter in S phase and that the C-terminal activation domains of E2F1, E2F4, and E2F5, when fused to the Gal4 DNA binding domain, are sufficient to specify position-dependent activation. To further investigate the role of activation in dhfr regulation, we tested other transactivation domains for their ability to activate the dhfr promoter. We found that the N-terminal transactivation domain of VP16 cannot activate the dhfr promoter. We propose that, unlike other E2F-regulated promoters, robust transcription from the dhfr promoter requires an E2F transactivation domain close to the transcription start site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.