ArticleMolecular and cellular biology1997
DNA-binding specificity of Mcm1: operator mutations that alter DNA-bending and transcriptional activities by a MADS box protein.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 63 citations in OpenAlex.
- Article
- Intrinsic cooperativity potentiates paralleleLife · 2018Article
- Contribution of transcription factor binding site motif variants to condition-specific gene expression patterns in budding yeast.PloS one · 2012Article
- Impact of DNA-binding position variants on yeast gene expression.Nucleic acids research · 2009Article
- Global mapping of protein-DNA interactions in vivo by digital genomic footprinting.Nature methods · 2009Article
- Single nucleotide changes in the human Igamma1 and Igamma4 promoters underlie different transcriptional responses to CD40.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Machine learning for regulatory analysis and transcription factor target prediction in yeast.Systems and synthetic biology · 2007Article
- Characterization of the plant Notchless homolog, a WD repeat protein involved in seed development.Plant molecular biology · 2006Article
- Metabolic-state-dependent remodeling of the transcriptome in response to anoxia and subsequent reoxygenation in Saccharomyces cerevisiae.Eukaryotic cell · 2006Article
- MAL and ternary complex factor use different mechanisms to contact a common surface on the serum response factor DNA-binding domain.Molecular and cellular biology · 2006Article
- N-terminal arm of Mcm1 is required for transcription of a subset of genes involved in maintenance of the cell wall.Eukaryotic cell · 2005Article
- Combined analysis of expression data and transcription factor binding sites in the yeast genome.BMC genomics · 2004Article
- Mcm1 promotes replication initiation by binding specific elements at replication origins.Molecular and cellular biology · 2004Article
- Sequence elements necessary for transcriptional activation of BAD1 in the yeast phase of Blastomyces dermatitidis.Eukaryotic cell · 2004Article
- Alpha1-induced DNA bending is required for transcriptional activation by the Mcm1-alpha1 complex.Nucleic acids research · 2004Article
- Identifying combinatorial regulation of transcription factors and binding motifs.Genome biology · 2004Article
- Sfp1 plays a key role in yeast ribosome biogenesis.Eukaryotic cell · 2003Article
- Position specific variation in the rate of evolution in transcription factor binding sites.BMC evolutionary biology · 2003Article
- The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region.Nucleic acids research · 2003Article
- Regulatory elements of the floral homeotic gene AGAMOUS identified by phylogenetic footprinting and shadowing.The Plant cell · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
The yeast Mcm1 protein is a member of the MADS box family of transcriptional regulatory factors, a class of DNA-binding proteins found in such diverse organisms as yeast, plants, flies, and humans. To explore the protein-DNA interactions of Mcm1 in vivo and in vitro, we have introduced an extensive series of base pair substitutions into an Mcm1 operator site and examined their effects on Mcm1-mediated transcriptional regulation and DNA-binding affinity. Our results show that Mcm1 uses a mechanism to contact the DNA that has some significant differences from the one used by the human serum response factor (SRF), a closely related MADS box protein in which the three-dimensional structure has been determined. One major difference is that 5-bromouracil-mediated photo-cross-linking experiments indicate that Mcm1 is in close proximity to functional groups in the major groove at the center of the recognition site whereas the SRF protein did not exhibit this characteristic. A more significant difference is that mutations at a position outside of the conserved CC(A/T)6GG site significantly reduce Mcm1-dependent DNA bending, while these substitutions have no effect on DNA bending by SRF. This result shows that the DNA bending by Mcm1 is sequence dependent and that the base-specific requirements for bending differ between Mcm1 and SRF. Interestingly, although these substitutions have a large effect on DNA bending and transcriptional activation by Mcm1, they have a relatively small effect on the DNA-binding affinity of the protein. This result suggests that the degree of DNA bending is important for transcriptional activation by Mcm1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.