ArticleMolecular and cellular biology1997
The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 110 citations in OpenAlex.
- Quantitative limits of host-driven HIV transcription and host gene control by the viral transactivator Tat.Nucleic acids research · 2026Article
- HIV Expression in Infected T Cell Clones.Viruses · 2024Review
- Single-cell transcriptional landscapes reveal HIV-1-driven aberrant host gene transcription as a potential therapeutic target.Science translational medicine · 2020Article
- A review on computational systems biology of pathogen-host interactions.Frontiers in microbiology · 2015Review
- Characterization of the influence of mediator complex in HIV-1 transcription.The Journal of biological chemistry · 2014Article
- Viral-host interactions that control HIV-1 transcriptional elongation.Chemical reviews · 2013Review
- Identification of cellular proteins required for replication of human immunodeficiency virus type 1.AIDS research and human retroviruses · 2012Article
- The control of HIV transcription: keeping RNA polymerase II on track.Cell host & microbe · 2011Review
- Transcription factor effector domains.Sub-cellular biochemistry · 2011Article
- Kick-sTARting HIV-1 transcription elongation by 7SK snRNP deporTATion.Nature structural & molecular biology · 2010Article
- RNA-mediated displacement of an inhibitory snRNP complex activates transcription elongation.Nature structural & molecular biology · 2010Article
- A versatile viral system for expression and depletion of proteins in mammalian cells.PloS one · 2009Article
- 9-Aminoacridine inhibition of HIV-1 Tat dependent transcription.Virology journal · 2009Article
- In vitro nuclear interactome of the HIV-1 Tat protein.Retrovirology · 2009Article
- Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous gene.Retrovirology · 2008Article
- Targeting tat inhibitors in the assembly of human immunodeficiency virus type 1 transcription complexes.Journal of virology · 2008Article
- HIV-1 Tat inhibits NGF-induced Egr-1 transcriptional activity and consequent p35 expression in neural cells.Journal of cellular physiology · 2008Article
- Inhibition of HIV-1 replication by P-TEFb inhibitors DRB, seliciclib and flavopiridol correlates with release of free P-TEFb from the large, inactive form of the complex.Retrovirology · 2007Article
- The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention.Medicinal research reviews · 2006Review
- Effect of SWI/SNF chromatin remodeling complex on HIV-1 Tat activated transcription.Retrovirology · 2006Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human immunodeficiency virus (HIV) encodes a transcriptional transactivator (Tat), which binds to an RNA hairpin called the transactivation response element (TAR) that is located downstream of the site of initiation of viral transcription. Tat stimulates the production of full-length viral transcripts by RNA polymerase II (pol II). In this study, we demonstrate that Tat coimmunoprecipitates with the pol II holoenzyme in cells and that it binds to the purified holoenzyme in vitro. Furthermore, Tat affinity chromatography purifies a holoenzyme from HeLa nuclear extracts which, upon addition of TBP and TFIIB, supports Tat transactivation in vitro, indicating that it contains all the cellular proteins required for the function of Tat. By demonstrating that Tat interacts with the holoenzyme in the absence of TAR, our data suggest a single-step assembly of Tat and the transcription complex on the long terminal repeat of HIV.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.