ArticleInfection and immunity1997
Molecular characterization of pldA, the structural gene for a phospholipase A from Campylobacter coli, and its contribution to cell-associated hemolysis.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 103 citations in OpenAlex.
- Pathogenicity assessment of Arcobacter butzleri isolated from Canadian agricultural surface water.BMC microbiology · 2024Article
- Comparative Analysis of Aerotolerance, Antibiotic Resistance, and Virulence Gene Prevalence inMicroorganisms · 2019Article
- Identification of Campylobacter fetus subsp. venerealis virulence genes in cervical mucus from cows.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Heme Uptake and Utilization by Gram-Negative Bacterial Pathogens.Frontiers in cellular and infection microbiology · 2019Review
- In silico proteomic and phylogenetic analysis of the outer membrane protein repertoire of gastric Helicobacter species.Scientific reports · 2018Article
- Differential Survival of Hyper-AerotolerantFrontiers in microbiology · 2017Article
- Epidemiological relationships of Campylobacter jejuni strains isolated from humans and chickens in South Korea.Journal of microbiology (Seoul, Korea) · 2017Article
- The outer membrane phospholipase A is essential for membrane integrity and type III secretion in Shigella flexneri.Open biology · 2016Article
- Toll-Like Receptor-4 Dependent Intestinal Gene Expression During Arcobacter Butzleri Infection of Gnotobiotic Il-10 Deficient Mice.European journal of microbiology & immunology · 2016Article
- Survey of small intestinal and systemic immune responses following murine Arcobacter butzleri infection.Gut pathogens · 2015Article
- Article
- Characterization of the Vibrio cholerae VolA surface-exposed lipoprotein lysophospholipase.Journal of bacteriology · 2014Article
- Identification of a functional type VI secretion system in Campylobacter jejuni conferring capsule polysaccharide sensitive cytotoxicity.PLoS pathogens · 2013Article
- Epidemiological association of Campylobacter jejuni groups with pathogenicity-associated genetic markers.BMC microbiology · 2012Article
- Interleukin-8 is the single most up-regulated gene in whole genome profiling of H. pylori exposed gastric epithelial cells.BMC microbiology · 2012Article
- Phospholipases a in trypanosomatids.Enzyme research · 2011Article
- Campylobacter jejuni isolates in Finnish patients differ according to the origin of infection.Gut pathogens · 2010Article
- Lipids in host-pathogen interactions: pathogens exploit the complexity of the host cell lipidome.Progress in lipid research · 2010Review
- The hemolytic and cytolytic activities of Serratia marcescens phospholipase A (PhlA) depend on lysophospholipid production by PhlA.BMC microbiology · 2009Article
- Structural biology of membrane-intrinsic beta-barrel enzymes: sentinels of the bacterial outer membrane.Biochimica et biophysica acta · 2008Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A gene (pldA) encoding a 35.0-kDa protein with significant homology to the Escherichia coli outer membrane phospholipase was identified upstream of an operon encoding an enterochelin transport system in Campylobacter coli. The results of this study suggest that this gene encodes an outer membrane phospholipase A in C. coli. First, expression of the pldA gene product in a PldA-deficient mutant of E. coli led to the restoration of phospholipase A activity. The recombinant product also partitioned to the outer membrane, suggesting that it may be similarly located in C. coli. Second, heterologous overexpression in E. coli, followed by in vitro folding and purification of C. coli PldA, resulted in pure protein which displayed calcium-dependent lysophospholipase and phospholipase A activities in vitro. Finally, mutants of C. coli in which the pldA gene had been inactivated by allelic exchange were deficient in phospholipase A activity. Phospholipases are associated with lysis of erythrocytes by a number of bacterial pathogens. The pldA mutant was shown to have a reduced hemolytic activity compared to the wild-type strain, suggesting a role for the phospholipase A in the lysis of erythrocytes by C. coli. Since hemolysins are intimately associated with the disease-causing potential of a number of bacterial pathogens, it is likely that the phospholipase A plays some role in Campylobacter virulence.
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