ArticleThe EMBO journal1997
Folate biosynthesis in higher plants: purification and molecular cloning of a bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase localized in mitochondria.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Detection of Sulfonamide Antibiotics Using an Elastic Hydrogel Microparticles-Based Optical Biosensor.ACS applied materials & interfaces · 2024Article
- Hypothetical Regulation of Folate Biosynthesis and Strategies for Folate Overproduction in Lactic Acid Bacteria.Preventive nutrition and food science · 2023Review
- Effect of Amino Acids on Folates Accumulation in Wheat Seedlings during Germination under Red Light Radiation.Molecules (Basel, Switzerland) · 2022Article
- Crystal structure of Arabidopsis thaliana HPPK/DHPS, a bifunctional enzyme and target of the herbicide asulam.Plant communications · 2022Article
- Antioxidants in Potatoes: A Functional View on One of the Major Food Crops Worldwide.Molecules (Basel, Switzerland) · 2021Review
- Influence of Sulfonamide Contamination Derived from Veterinary Antibiotics on Plant Growth and Development.Antibiotics (Basel, Switzerland) · 2020Article
- Evolution of folate biosynthesis and metabolism across algae and land plant lineages.Scientific reports · 2019Article
- A highly efficient sulfadiazine selection system for the generation of transgenic plants and algae.Plant biotechnology journal · 2019Article
- Transcriptional and Post-transcriptional Regulation of Organellar Gene Expression (OGE) and Its Roles in Plant Salt Tolerance.International journal of molecular sciences · 2019Review
- Discovery of the fourth mobile sulfonamide resistance gene.Microbiome · 2017Article
- Tetrahydrofolate Modulates Floral Transition through Epigenetic Silencing.Plant physiology · 2017Article
- Folates in Plants: Research Advances and Progress in Crop Biofortification.Frontiers in chemistry · 2017Review
- Binding Isotope Effects for para-Aminobenzoic Acid with Dihydropteroate Synthase from Staphylococcus aureus and Plasmodium falciparum.ACS chemical biology · 2015Article
- Overexpression of folate biosynthesis genes in rice (Oryza sativa L.) and evaluation of their impact on seed folate content.Plant foods for human nutrition (Dordrecht, Netherlands) · 2014Article
- Article
- Catalysis and sulfa drug resistance in dihydropteroate synthase.Science (New York, N.Y.) · 2012Article
- Pulsing of membrane potential in individual mitochondria: a stress-induced mechanism to regulate respiratory bioenergetics in Arabidopsis.The Plant cell · 2012Article
- Integrative identification of Arabidopsis mitochondrial proteome and its function exploitation through protein interaction network.PloS one · 2011Article
- Metabolism of the folate precursor p-aminobenzoate in plants: glucose ester formation and vacuolar storage.The Journal of biological chemistry · 2008Article
- Dual targeting of Arabidopsis holocarboxylase synthetase1: a small upstream open reading frame regulates translation initiation and protein targeting.Plant physiology · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In pea leaves, the synthesis of 7,8-dihydropteroate, a primary step in folate synthesis, was only detected in mitochondria. This reaction is catalyzed by a bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase enzyme, which represented 0.04-0.06% of the matrix proteins. The enzyme had a native mol. wt of 280-300 kDa and was made up of identical subunits of 53 kDa. The reaction catalyzed by the 7,8-dihydropteroate synthase domain of the protein was Mg2+-dependent and behaved like a random bireactant system. The related cDNA contained an open reading frame of 1545 bp and the deduced amino acid sequence corresponded to a polypeptide of 515 residues with a calculated M(r) of 56,454 Da. Comparison of the deduced amino acid sequence with the N-terminal sequence of the purified protein indicated that the plant enzyme is synthesized with a putative mitochondrial transit peptide of 28 amino acids. The calculated M(r) of the mature protein was 53,450 Da. Southern blot experiments suggested that a single-copy gene codes for the enzyme. This result, together with the facts that the protein is synthesized with a mitochondrial transit peptide and that the activity was only detected in mitochondria, strongly supports the view that mitochondria is the major (unique?) site of 7,8-dihydropteroate synthesis in higher plant cells.
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