ArticleThe EMBO journal1997
Functional characterization of the fission yeast Start-specific transcription factor Res2.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.G3 (Bethesda, Md.) · 2022Article
- Recent insights into mechanisms preventing ectopic centromere formation.Open biology · 2021Review
- Transcription Activator Swi6 Interacts with Mbp1 inJournal of fungi (Basel, Switzerland) · 2021Article
- Down-regulation of Cdk1 activity in G1 coordinates the G1/S gene expression programme with genome replication.Current genetics · 2019Review
- Cell Cycle-Regulated Transcription of CENP-A by the MBF Complex Ensures Optimal Level of CENP-A for Centromere Formation.Genetics · 2019Article
- Evolution of asexual and sexual reproduction in the aspergilli.Studies in mycology · 2018Article
- Set2 Methyltransferase Facilitates DNA Replication and Promotes Genotoxic Stress Responses through MBF-Dependent Transcription.Cell reports · 2017Article
- The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species.Microbiology and molecular biology reviews : MMBR · 2016Review
- Structural basis of DNA recognition by PCG2 reveals a novel DNA binding mode for winged helix-turn-helix domains.Nucleic acids research · 2015Article
- Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.PloS one · 2013Article
- G1/S transcription factor orthologues Swi4p and Swi6p are important but not essential for cell proliferation and influence hyphal development in the fungal pathogen Candida albicans.Eukaryotic cell · 2011Article
- Phosphorylation of the MBF repressor Yox1p by the DNA replication checkpoint keeps the G1/S cell-cycle transcriptional program active.PloS one · 2011Article
- Redundant mechanisms prevent mitotic entry following replication arrest in the absence of Cdc25 hyper-phosphorylation in fission yeast.PloS one · 2011Article
- The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.PLoS genetics · 2009Article
- The cell cycle-regulated genes of Schizosaccharomyces pombe.PLoS biology · 2005Article
- DSC1-MCB regulation of meiotic transcription in Schizosaccharomyces pombe.Molecular genetics and genomics : MGG · 2004Article
- A sequence element downstream of the yeast HTB1 gene contributes to mRNA 3' processing and cell cycle regulation.Molecular and cellular biology · 2002Article
- The G(1) cyclin Cln3 promotes cell cycle entry via the transcription factor Swi6.Molecular and cellular biology · 2002Article
- A role for the START gene-specific transcription factor complex in the inactivation of cyclin B and Cut2 destruction.Molecular biology of the cell · 2000Article
- Cell cycle-regulated transcription in fission yeast: Cdc10-Res protein interactions during the cell cycle and domains required for regulated transcription.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the fission yeast Schizosaccharomyces pombe, transcriptional activation at Start is mediated by complexes that bind the MCB. Two such complexes have been identified; both contain the Cdc10 protein in partnership with either the Res1 or Res2 protein. Characterization of null mutants suggests that the Res1-Cdc10 complex predominantly functions in mitotic cells whereas the Res2-Cdc10 complex is required for meiosis and spore formation. Here we have characterized the functional domains of the Res2 protein. The N-terminus is both necessary and sufficient for DNA binding, whereas the C-terminus is the region involved in the interaction with the Cdc10 protein. The centrally located ankyrin repeats are dispensable for both functions. Res2 binds to DNA as a dimer. In addition, complexes containing both Res1 and Res2 can form and bind to DNA in vitro. Furthermore, the major MCB-specific complex detected in extracts from wild-type cells contains Res1 and Res2; the complex is lost when either gene is deleted and can be recognized by antibodies specific to both proteins. In order to understand the basis for the specific function of Res2 in meiosis, hybrids between Res1 and Res2 were constructed and their functions analysed. The results indicate an absolute requirement for the Res2 C-terminus for normal meiosis to occur whereas the origin of the DNA-binding region is irrelevant. The implications of these results for the regulation of the MCB-binding complexes will be discussed.
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