ArticleNucleic acids research1997
Precise branch point mapping and quantification of splicing intermediates.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
64 citing papers in PubMed, 90 citations in OpenAlex.
- Professor Thomas Börner: the pioneer of molecular plant genetics in East Germany turns 80!Molecular genetics and genomics : MGG · 2026Article
- The debranching enzyme Dbr1 regulates lariat turnover and intron splicing.Nature communications · 2024Article
- Variations of intronic branchpoint motif: identification and functional implications in splicing and disease.Communications biology · 2023Review
- Recursive splicing discovery using lariats in total RNA sequencing.Life science alliance · 2023Article
- In Silico Identification and Characterization of circRNAs as Potential Virulence-Related miRNA/siRNA Sponges fromNon-coding RNA · 2022Article
- Promoter-Bound Full-Length Intronic Circular RNAs-RNA Polymerase II Complexes Regulate Gene Expression in the Human ParasiteNon-coding RNA · 2022Article
- Article
- Detecting circRNA in purified spliceosomal P complex.Methods (San Diego, Calif.) · 2021Article
- Restriction of an intron size en route to endothermy.Nucleic acids research · 2021Article
- HPV18 Utilizes Two Alternative Branch Sites for E6*I Splicing to Produce E7 Protein.Virologica Sinica · 2019Article
- Dual coding potential of a 2',5'-branched ribonucleotide in DNA.RNA (New York, N.Y.) · 2019Article
- ShapeShifter: a novel approach for identifying and quantifying stable lariat intronic species in RNAseq data.Quantitative biology (Beijing, China) · 2018Article
- Most human introns are recognized via multiple and tissue-specific branchpoints.Genes & development · 2018Article
- Postsplicing-Derived Full-Length Intron Circles in the Protozoan ParasiteFrontiers in cellular and infection microbiology · 2018Article
- A heuristic model for computational prediction of human branch point sequence.BMC bioinformatics · 2017Article
- Arm-specific cleavage and mutation during reverse transcription of 2΄,5΄-branched RNA by Moloney murine leukemia virus reverse transcriptase.Nucleic acids research · 2017Article
- Large-scale analysis of branchpoint usage across species and cell lines.Genome research · 2017Article
- Identification of new branch points and unconventional introns in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2016Article
- Sequential splicing of a group II twintron in the marine cyanobacterium Trichodesmium.Scientific reports · 2015Article
- Selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) for direct, versatile and accurate RNA structure analysis.Nature protocols · 2015Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lariat intermediates of a group II intron were investigated via RT-PCR. Several reverse transcriptases appeared capable of reading through a branched nucleotide. A new method has been established that yields precise information about the location of the branch point within an intron. As an extension of our approach, antisense transcripts of the previously cloned PCR products were successfully used in RNase Protection Assays, providing a tool for quantification of splicing intermediates. Application of the method presented to other self-splicing introns as well as introns in nuclear pre-mRNAs is envisaged.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.