ArticleNucleic acids research1997
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) of endonuclease digests of RNA.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Characterization and Sequence Mapping of Large RNA and mRNA Therapeutics Using Mass Spectrometry.Analytical chemistry · 2022Article
- A general LC-MS-based RNA sequencing method for direct analysis of multiple-base modifications in RNA mixtures.Nucleic acids research · 2019Article
- High resolution fingerprinting of single and double-stranded RNA using ion-pair reverse-phase chromatography.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2019Article
- Novel ribonuclease activity of cusativin from Cucumis sativus for mapping nucleoside modifications in RNA.Analytical and bioanalytical chemistry · 2017Article
- Purification and characterisation of dsRNA using ion pair reverse phase chromatography and mass spectrometry.Journal of chromatography. A · 2017Article
- On the mechanism of RNA phosphodiester backbone cleavage in the absence of solvent.Nucleic acids research · 2015Article
- Elucidating the higher-order structure of biopolymers by structural probing and mass spectrometry: MS3D.Journal of mass spectrometry : JMS · 2010Article
- Identification of RNA molecules by specific enzyme digestion and mass spectrometry: software for and implementation of RNA mass mapping.Nucleic acids research · 2009Article
- Electrospray ionisation-cleavable tandem nucleic acid mass tag-peptide nucleic acid conjugates: synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS.Nucleic acids research · 2007Article
- DNA sequencing by MALDI-TOF MS using alkali cleavage of RNA/DNA chimeras.Nucleic acids research · 2007Article
- A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Nucleic acids research · 2006Article
- Bacterial genotyping by 16S rRNA mass cataloging.BMC bioinformatics · 2006Article
- Microbial identification by mass cataloging.BMC bioinformatics · 2006Article
- Reverse Sanger sequencing of RNA by MALDI-TOF mass spectrometry after solid phase purification.Nucleic acids research · 2004Article
- MALDI-TOF mass spectrometry: a versatile tool for high-performance DNA analysis.Molecular biotechnology · 2004Review
- Rapid and accurate characterisation of short tandem repeats by MALDI-TOF analysis of endonuclease cleaved RNA transcripts.Nucleic acids research · 2004Article
- Fast and accurate determination of sites along the FUT2 in vitro transcript that are accessible to antisense oligonucleotides by application of secondary structure predictions and RNase H in combination with MALDI-TOF mass spectrometry.Nucleic acids research · 2003Article
- RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis.Nucleic acids research · 2003Article
- RNaseCut: a MALDI mass spectrometry-based method for SNP discovery.Nucleic acids research · 2003Article
- The exo- or endonucleolytic preference of bovine pancreatic ribonuclease A depends on its subsites structure and on the substrate size.Protein science : a publication of the Protein Society · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The determination of RNA sequences using base- specific enzymatic cleavages is a well established method. Different synthetic RNA molecules were analyzed for uniformity of degradation by RNase T1, U2, A and PhyM under reaction conditions compatible with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS), to identify the positions of G, A and pyrimidine residues. In order to get a complete set of fragments derived from cleavage at every phosphodiester bond, the samples were also subjected to a limited alkaline hydrolysis. Additionally, the 5'-terminus fragments of a 49mer RNA transcript were isolated by way of 5'-biotinylation and streptavidin-coated magnetic beads (Dynal), followed by a RNase U2digestion. MALDI-MS of the generated fragments is presented as an efficient technique for a direct read out of the nucleotide sequence.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.