ArticleJournal of clinical microbiology1997
Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NM.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
77 citing papers in PubMed, 178 citations in OpenAlex.
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- Determination of Effective Prophylactic Responses of Truncated Flagellin Protein as a Vaccine Candidate Against UropathogenicInternational journal of microbiology · 2026Article
- Characterization of a New TemperatePathogens (Basel, Switzerland) · 2022Article
- Genetic diversity of the intimin gene (eae) in non-O157 Shiga toxin-producing Escherichia coli strains in China.Scientific reports · 2020Article
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- New Approaches forPathogens (Basel, Switzerland) · 2020Article
- A large self-transmissible resistance plasmid from Nigeria contains genes that ameliorate a carrying cost.Scientific reports · 2019Article
- Escherichia coli H-Genotyping PCR: a Complete and Practical Platform for Molecular H Typing.Journal of clinical microbiology · 2018Article
- Genotypic and Phenotypic Characteristics Associated with Biofilm Formation by Human Clinical Escherichia coli Isolates of Different Pathotypes.Applied and environmental microbiology · 2017Article
- Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli.BMC microbiology · 2017Article
- High Prevalence of Virulence Genes in Specific Genotypes of Atypical EnteropathogenicFrontiers in cellular and infection microbiology · 2017Article
- Classes 1 and 2 integrons in faecal Escherichia coli strains isolated from mother-child pairs in Nigeria.PloS one · 2017Article
- Phylogenetic Analysis of Enteroaggregative Escherichia coli (EAEC) Isolates from Japan Reveals Emergence of CTX-M-14-Producing EAEC O25:H4 Clones Related to Sequence Type 131.Journal of clinical microbiology · 2016Article
- Diarrhoeagenic Escherichia coli in mother-child Pairs in Ile-Ife, South Western Nigeria.BMC infectious diseases · 2016Article
- Development of a Sandwich ELISA for EHEC O157:H7 Intimin γ1.PloS one · 2016Article
- Molecular and Phylogenetic Characterization of Non-O157 Shiga Toxin-ProducingFrontiers in cellular and infection microbiology · 2016Article
- Shiga Toxin-Producing Escherichia coli in Plateau Pika (Ochotona curzoniae) on the Qinghai-Tibetan Plateau, China.Frontiers in microbiology · 2016Article
- Genetic Diversity of Intimin Gene of Atypical Enteropathogenic Escherichia coli Isolated from Human, Animals and Raw Meats in China.PloS one · 2016Article
- Flagellin and GroEL mediates in vitro binding of an atypical enteropathogenic Escherichia coli to cellular fibronectin.BMC microbiology · 2015Article
- Rapid and Easy In Silico Serotyping of Escherichia coli Isolates by Use of Whole-Genome Sequencing Data.Journal of clinical microbiology · 2015Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent outbreaks of disease caused by Escherichia coli O157:H7 have focused much attention on this newly emerged pathogen. Identification of the H7 flagellar antigen is critical for the confirmation of E. coli O157:H7; however, clinical isolates are frequently nonmotile and do not produce detectable H antigen. To further characterize nonmotile isolates (designated NM), we developed a PCR-restriction fragment length polymorphism (PCR-RFLP) test to identify and characterize the gene encoding the H antigen (fliC) in E. coli. The entire coding sequence of fliC was amplified by PCR, the amplicon was restricted with RsaI, and the restriction fragment pattern was examined after gel electrophoresis. Two hundred eighty E. coli isolates representing serotypes O157:H7 and O157:NM, flagellar antigen H7 groups associated with other O serogroups, and all other flagellar antigen groups were analyzed. A single restriction pattern (pattern A) was identified for O157:H7 isolates, O157:NM isolates that produced Shiga toxin (formerly Shiga-like toxin or verotoxin), and 16 of 18 O55:H7 isolates. Flagellar antigen group H7 isolates of non-O157 serotypes had one of three banding patterns distinct from pattern A. A wide variety of patterns were found among isolates of the other 52 flagellar antigen groups; however, none was identical to the O157:H7 pattern. Thirteen of 15 nonmotile strains that did not produce the A pattern had patterns that matched those of other known H groups. The PCR-RFLP in conjunction with O serogroup determination will be useful in identifying E. coli O157:H7 and related strains that do not express immunoreactive H antigen and could be expanded to include other clinically important E. coli strains.
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