ArticleProceedings of the National Academy of Sciences of the United States of America1997
A membrane-anchored E-type endo-1,4-beta-glucanase is localized on Golgi and plasma membranes of higher plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 140 citations in OpenAlex.
- Plant Defense Proteins: Recent Discoveries and Applications.Plants (Basel, Switzerland) · 2025Review
- Identifying the role of cellulase geneMolecular breeding : new strategies in plant improvement · 2025Article
- Genetic Evidence ofGenes · 2024Article
- Mutation in the Endo-β-1,4-glucanase (KORRIGAN) Is Responsible for Thick Leaf Phenotype in Sorghum.Plants (Basel, Switzerland) · 2022Article
- Emerging Roles of β-Glucanases in Plant Development and Adaptative Responses.Plants (Basel, Switzerland) · 2022Review
- In Silico Characterization and Functional Validation of Cell Wall Modification Genes Imparting Waterlogging Tolerance in Maize.Bioinformatics and biology insights · 2017Article
- In silico Identification and Taxonomic Distribution of Plant Class C GH9 Endoglucanases.Frontiers in plant science · 2016Article
- Down-regulation of the cotton endo-1,4-β-glucanase gene KOR1 disrupts endosperm cellularization, delays embryo development, and reduces early seedling vigour.Journal of experimental botany · 2015Article
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- Genes involved in floral meristem in tomato exhibit drastically reduced genetic diversity and signature of selection.BMC plant biology · 2014Article
- Characterization of Cryptopygus antarcticus endo-β-1,4-glucanase from Bombyx mori expression systems.Molecular biotechnology · 2014Article
- The Cellulase KORRIGAN Is Part of the Cellulose Synthase Complex.Plant physiology · 2014Article
- The jiaoyao1 Mutant Is an Allele of korrigan1 That Abolishes Endoglucanase Activity and Affects the Organization of Both Cellulose Microfibrils and Microtubules in Arabidopsis.The Plant cell · 2014Article
- KORRIGAN1 interacts specifically with integral components of the cellulose synthase machinery.PloS one · 2014Article
- Cloning and characterization of endo-β-1,4-glucanase genes in the common wheat line three pistils.Genetics and molecular biology · 2013Article
- Endo-(1,4)-β-glucanase gene families in the grasses: temporal and spatial co-transcription of orthologous genes.BMC plant biology · 2012Article
- Expression of Trichoderma viride endoglucanase III in the larvae of silkworm, Bombyx mori L. and characteristic analysis of the recombinant protein.Molecular biology reports · 2011Article
- Biochemistry of fruit softening: an overview.Physiology and molecular biology of plants : an international journal of functional plant biology · 2009Article
- Expression of endo-1,4-beta-glucanase (cel1) in Arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening.Plant cell reports · 2006Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endo-1,4-beta-D-glucanases (EGases, EC 3.2.1.4) are enzymes produced in bacteria, fungi, and plants that hydrolyze polysaccharides possessing a 1,4-beta-D-glucan backbone. All previously identified plant EGases are E-type endoglucanases that possess signal sequences for endoplasmic reticulum entry and are secreted to the cell wall. Here we report the characterization of a novel E-type plant EGase (tomato Cel3) with a hydrophobic transmembrane domain and structure typical of type II integral membrane proteins. The predicted protein is composed of 617 amino acids and possesses seven potential sites for N-glycosylation. Cel3 mRNA accumulates in young vegetative tissues with highest abundance during periods of rapid cell expansion, but is not hormonally regulated. Antibodies raised to a recombinant Cel3 protein specifically recognized three proteins, with apparent molecular masses of 93, 88, and 53 kDa, in tomato root microsomal membranes separated by sucrose density centrifugation. The 53-kDa protein comigrated in the gradient with plasma membrane markers, the 88-kDa protein with Golgi membrane markers, and the 93-kDa protein with markers for both Golgi and plasma membranes. EGase enzyme activity was also found in regions of the density gradient corresponding to both Golgi and plasma membranes, suggesting that Cel3 EGase resides in both membrane systems, the sites of cell wall polymer biosynthesis. The in vivo function of Cel3 is not known, but the only other known membrane-anchored EGase is present in Agrobacterium tumefaciens where it is required for cellulose biosynthesis.
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