ArticleProceedings of the National Academy of Sciences of the United States of America1997
Transient and selectable transformation of the parasitic protist Trichomonas vaginalis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 87 citations in OpenAlex.
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- Targeting histone acetylation to overcome drug resistance in the parasitebioRxiv : the preprint server for biology · 2025Article
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- VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles.Cellular and molecular life sciences : CMLS · 2021Article
- Ultrastructural and Functional Analysis of a Novel Extra-Axonemal Structure in Parasitic Trichomonads.Frontiers in cellular and infection microbiology · 2021Article
- Determinants of translation efficiency in the evolutionarily-divergent protist Trichomonas vaginalis.BMC molecular and cell biology · 2020Article
- Characterization of the BspA and Pmp protein family of trichomonads.Parasites & vectors · 2019Article
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- The ProtozoanmBio · 2018Article
- Protein Palmitoylation Plays an Important Role inMolecular & cellular proteomics : MCP · 2018Article
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- Membrane-shed vesicles from the parasite Trichomonas vaginalis: characterization and their association with cell interaction.Cellular and molecular life sciences : CMLS · 2018Article
- CRISPR/Cas9-mediated gene modification and gene knock out in the human-infective parasite Trichomonas vaginalis.Scientific reports · 2018Article
- Auranofin inactivates Trichomonas vaginalis thioredoxin reductase and is effective against trichomonads in vitro and in vivo.International journal of antimicrobial agents · 2016Article
- N-Terminal Presequence-Independent Import of Phosphofructokinase into Hydrogenosomes of Trichomonas vaginalis.Eukaryotic cell · 2015Article
- A Trichomonas vaginalis Rhomboid Protease and Its Substrate Modulate Parasite Attachment and Cytolysis of Host Cells.PLoS pathogens · 2015Article
- The parasite Trichomonas vaginalis expresses thousands of pseudogenes and long non-coding RNAs independently from functional neighbouring genes.BMC genomics · 2014Article
- Trichomonas vaginalis homolog of macrophage migration inhibitory factor induces prostate cell growth, invasiveness, and inflammatory responses.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Novel functions of an iron-sulfur flavoprotein from Trichomonas vaginalis hydrogenosomes.Antimicrobial agents and chemotherapy · 2014Article
- A dynamin-related protein contributes to Trichomonas vaginalis hydrogenosomal fission.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have developed methods to transiently and selectably transform the human-infective protist Trichomonas vaginalis. This parasite, a common cause of vaginitis worldwide, is one of the earlier branching eukaryotes studied to date. We have introduced three heterologous genes into T. vaginalis by electroporation and have used the 5' and 3' untranslated regions of the endogenous gene alpha-succinyl CoA synthetase B (alpha-SCSB) to drive transcription of these genes. Transient expression of two reporter proteins, chloramphenicol acetyltransferase (CAT) or luciferase, was detected when electroporating in the presence of 50 microg closed-circular construct. Optimal levels of expression were observed using approximately 2.5 x 10(8) T. vaginalis cells and 350 volts, 960 microFd for electroporation; however, other conditions also led to significant reporter gene expression. A time course following the expression of CAT in T. vaginalis transient transformants revealed the highest level of expression 8-21 hr postelectroporation and showed that CAT activity is undetectable using TLC by 99 hr postelectroporation. The system we established to obtain selectable transformants uses the neomycin phosphotransferase (neo) gene as the selectable marker. Cells electroporated with 20 microg of the NEO construct were plated in the presence of 50 microg/ml paromomycin and incubated in an anaerobic chamber. The paromomycin-resistant colonies that formed within 3-5 days were cultivated in the presence of drug and DNA was isolated for analyses. The NEO construct was shown to be maintained episomally, as a closed-circle, at between 10-30 copies per cell. The ability to transiently and selectably transform T. vaginalis should greatly enhance research on this important human parasite.
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