ArticleProceedings of the National Academy of Sciences of the United States of America1997
Obese gene expression at in vivo levels by fat pads derived from s.c. implanted 3T3-F442A preadipocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 145 citations in OpenAlex.
- Effects of mifepristone on adipocyte differentiation in mouse 3T3-L1 cells.Cellular & molecular biology letters · 2024Article
- Transplantation of committed pre-adipocytes from brown adipose tissue improves whole-body glucose homeostasis.iScience · 2024Article
- Early enforcement of cell identity by a functional component of the terminally differentiated state.PLoS biology · 2022Article
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- WAT-on-a-chip integrating human mature white adipocytes for mechanistic research and pharmaceutical applications.Scientific reports · 2020Article
- Dysregulation of a long noncoding RNA reduces leptin leading to a leptin-responsive form of obesity.Nature medicine · 2019Article
- Mouse Embryonic Fibroblasts Protect ob/ob Mice From Obesity and Metabolic Complications.Endocrinology · 2018Article
- A noncanonical PPARγ/RXRα-binding sequence regulates leptin expression in response to changes in adipose tissue mass.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Microfluidic systems for studying dynamic function of adipocytes and adipose tissue.Analytical and bioanalytical chemistry · 2018Review
- PPARγ neddylation essential for adipogenesis is a potential target for treating obesity.Cell death and differentiation · 2016Article
- Identification of zinc finger protein Bcl6 as a novel regulator of early adipose commitment.Open biology · 2016Article
- Identification of Mouse Mesenteric and Subcutaneous in vitro Adipogenic Cells.Scientific reports · 2016Article
- Nuclear Factor-Y is an adipogenic factor that regulates leptin gene expression.Molecular metabolism · 2015Article
- The Anti-Adipogenic Potential of COUP-TFII Is Mediated by Downregulation of the Notch Target Gene Hey1.PloS one · 2015Article
- Caveolin-1 expression and cavin stability regulate caveolae dynamics in adipocyte lipid store fluctuation.Diabetes · 2014Article
- Improved methodologies for the study of adipose biology: insights gained and opportunities ahead.Journal of lipid research · 2014Review
- Hepatocyte growth factor regulates neovascularization in developing fat pads.American journal of physiology. Endocrinology and metabolism · 2014Article
- Transcription factor Snail is a novel regulator of adipocyte differentiation via inhibiting the expression of peroxisome proliferator-activated receptor γ.Cellular and molecular life sciences : CMLS · 2013Article
- Protein kinase C δ (PKCδ) splice variants modulate apoptosis pathway in 3T3L1 cells during adipogenesis: identification of PKCδII inhibitor.The Journal of biological chemistry · 2013Article
- Local adipocytes enable estrogen-dependent breast cancer growth: Role of leptin and aromatase.Adipocyte · 2013Article
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
3T3-F442A preadipocytes implanted s.c. into athymic mice develop into fat pads that are indistinguishable from normal adipose tissue. Implanted preadipocytes harboring a beta-galactosidase transgene gave rise to fat pads in which almost all adipocytes expressed beta-galactosidase. This finding proved that the implanted 3T3-F442A preadipocytes, rather than endogenous preadipose cells, gave rise to the newly developed "adipose tissue." 3T3-F442A preadipocytes, when differentiated into adipocytes in cell culture, express the obese gene at an unexpectedly low level, i.e., </=1% the level in adipose tissue. However, adipose tissue derived from s.c. implanted 3T3-F442A preadipocytes expressed leptin mRNA at a level comparable to that in epididymal adipose tissue. These findings indicate that a factor(s) or condition, present in the tissue context and necessary for maximal obese gene expression, is lacking in cell culture. Furthermore, adipocytes derived from the implanted cells were hormonally responsive in that leptin mRNA levels were up-regulated 3- to 8-fold by glucocorticoid injection into the host animal. Thus, these findings indicate that adipose-specific promoter-reporter constructs, transfected into 3T3-F442A preadipocytes, can be tested in an in vivo context during and after development of these cells into adipose tissue. Furthermore, the effect of transgenes on the adipogenic development of the implanted preadipocytes can be assessed. Thus, this approach offers a faster and less costly alternative to the transgenic mouse method for assessing adipose gene function.
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