ArticlePlant physiology1997
Protein transport into higher plant peroxisomes. In vitro import assay provides evidence for receptor involvement.
Article in Plant physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Accurate and live peroxisome biogenesis evaluation achieved by lentiviral expression of a green fluorescent protein fused to a peroxisome targeting signal 1.Histochemistry and cell biology · 2020Article
- A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery.International journal of molecular sciences · 2019Review
- A cell-free organelle-based in vitro system for studying the peroxisomal protein import machinery.Nature protocols · 2016Article
- Covalent Label Transfer between Peroxisomal Importomer Components Reveals Export-driven Import Interactions.The Journal of biological chemistry · 2016Article
- Inheritance of the Md-ACS1 gene and its relationship to fruit softening in apple ( Malus x domestica Borkh.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
- Import of the peroxisomal targeting signal type 2 protein 3-ketoacyl-coenzyme a thiolase into glyoxysomes.Plant physiology · 2003Article
- Rapid identification of 1-aminocyclopropane-1-carboxylate (ACC) synthase genotypes in cultivars of Japanese pear (Pyrus pyrifolia Nakai) using CAPS markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2003Article
- Hydrogen peroxide homeostasis: activation of plant catalase by calcium/calmodulin.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Gene isolation and characterization of two acyl CoA oxidases from soybean with broad substrate specificities and enhanced expression in the growing seedling axis.Plant molecular biology · 2001Article
- Characterization of intermediates in the process of plant peroxisomal protein import.The EMBO journal · 1998Article
- Identification and analysis of the plant peroxisomal targeting signal 1 receptor NtPEX5.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The surprising complexity of peroxisome biogenesis.Plant molecular biology · 1998Review
- Nucleotide triphosphates are required for the transport of glycolate oxidase into peroxisomes.Plant physiology · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Peroxisome biogenesis requires that proteins be transported from their site of synthesis in the cytoplasm to their final location in the peroxisome matrix or membrane. Glyoxysomes are a class of peroxisomes found primarily in germinating seedlings and are involved in mobilizing fatty acids via the glyoxylate cycle and the beta-oxidation pathway. We have used an in vitro assay to study the mechanism(s) of import of proteins into glyoxysomes. Results from this assay indicate that the transport process is time- and temperature-dependent and is specific for peroxisomal proteins. Isocitrate lyase, a glyoxysomal protein, and the leaf-type peroxisomal enzyme glycolate oxidase (GLO) were transported into pumpkin (Cucurbita pepo) glyoxysomes with no apparent differences in efficiency of import. Thus, this in vitro assay appears to be physiologically relevant and correlates well with expected in vivo conditions. Protein import was also energy-dependent and saturable. Nonradiolabeled GLO competed with radiolabeled, in vitro-synthesized GLO for components of the import machinery. Finally, pretreatment of the isolated glyoxysomes with protease virtually abolished subsequent import of GLO. Taken together, these results indicate that a proteinaceous receptor is involved in the import of peroxisomal proteins.
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