ArticleMolecular and cellular biology1997
A yeast TATA-binding protein mutant that selectively enhances gene expression from weak RNA polymerase II promoters.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- TATA-binding protein variants that bypass the requirement for Mot1 in vivo.The Journal of biological chemistry · 2009Article
- The transcriptional repressor activator protein Rap1p is a direct regulator of TATA-binding protein.The Journal of biological chemistry · 2008Article
- Computer-based screening of functional conformers of proteins.PLoS computational biology · 2008Article
- A TATA binding protein regulatory network that governs transcription complex assembly.Genome biology · 2007Article
- The transcriptional activator GAL4-VP16 regulates the intra-molecular interactions of the TATA-binding protein.Journal of biosciences · 2003Article
- Structural and functional analysis of mutations along the crystallographic dimer interface of the yeast TATA binding protein.Molecular and cellular biology · 2003Article
- A TATA binding protein mutant with increased affinity for DNA directs transcription from a reversed TATA sequence in vivo.Molecular and cellular biology · 2002Article
- Functional interaction between Ssu72 and the Rpb2 subunit of RNA polymerase II in Saccharomyces cerevisiae.Molecular and cellular biology · 2000Article
- Analysis of cellular factors that mediate nuclear export of RNAs bearing the Mason-Pfizer monkey virus constitutive transport element.Journal of virology · 2000Article
- TATA-binding protein mutants that increase transcription from enhancerless and repressed promoters in vivo.Molecular and cellular biology · 2000Article
- Molecular genetics of the RNA polymerase II general transcriptional machinery.Microbiology and molecular biology reviews : MMBR · 1998Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a unique gain-of-function mutant of the TATA-binding protein (TBP) subunit of Saccharomyces cerevisiae TFIID that, at least in part, renders transcriptional transactivators dispensable for efficient mRNA expression. The yTBPN69S mutant enhances transcription from weaker yeast promoter elements by up to 50-fold yet does not significantly increase gene expression directed by highly active promoters. Therefore, this TBP mutant and transcriptional transactivators appear to affect a common rate-limiting step in transcription initiation. Consistent with the hypothesis that this step is TFIID recruitment, tethering of TBP to a target promoter via a heterologous DNA binding domain, which is known to bypass the need for transcriptional transactivators, also nullifies the enhancing effect exerted by the N69S mutation. These data provide genetic support for the hypothesis that TFIID recruitment represents a rate-limiting step in the initiation of mRNA transcription that is specifically enhanced by transcriptional transactivators.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.