ArticleMolecular and cellular biology1997
The repeat organizer, a specialized insulator element within the intergenic spacer of the Xenopus rRNA genes.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Suppressor of hairy-wing, modifier of mdg4 and centrosomal protein of 190 gene orthologues of the gypsy insulator complex in the malaria mosquito, Anopheles stephensi.Insect molecular biology · 2016Article
- Exogenous gypsy insulator sequences modulate transgene expression in the malaria vector mosquito, Anopheles stephensi.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A BEAF dependent chromatin domain boundary separates myoglianin and eyeless genes of Drosophila melanogaster.Nucleic acids research · 2011Article
- Dropout alignment allows homology recognition and evolutionary analysis of rDNA intergenic spacers.Journal of molecular evolution · 2008Article
- Intragenomic variation in ribosomal RNA gene of the sea urchin Lytechinus variegatus.Molecular biology reports · 2005Article
- Concerted and birth-and-death evolution of multigene families.Annual review of genetics · 2005Review
- A conserved sequence upstream of the B29 (Ig beta, CD79b) gene interacts with YY1.Molecular biology reports · 2004Article
- Transcription of endogenous and exogenous R2 elements in the rRNA gene locus of Drosophila melanogaster.Molecular and cellular biology · 2003Article
- Restricted chromosomal silencing in nucleolar dominance.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Assessing a role for enhancer-blocking activity in gene regulation within the murine T-cell receptor alpha/delta locus.Immunology · 2001Article
- Trypanosoma brucei variant surface glycoprotein regulation involves coupled activation/inactivation and chromatin remodeling of expression sites.The EMBO journal · 1999Article
- Functional sub-division of the Drosophila genome via chromatin looping: the emerging importance of CP190.Nucleus (Austin, Tex.)Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have identified a novel activity for the region of the intergenic spacer of the Xenopus laevis rRNA genes that contains the 35- and 100-bp repeats. We devised a new assay for this region by constructing DNA plasmids containing a tandem repeat of rRNA reporter genes that were separated by the 35- and 100-bp repeat region and a rRNA gene enhancer. When the 35- and 100-bp repeat region is present in its normal position and orientation at the 3' end of the rRNA reporter genes, the enhancer activates the adjacent downstream promoter but not the upstream rRNA promoter on the same plasmid. Because this element can restrict the range of an enhancer's activity in the context of tandem genes, we have named it the repeat organizer (RO). The ability to restrict enhancer action is a feature of insulator elements, but unlike previously described insulator elements the RO does not block enhancer action in a simple enhancer-blocking assay. Instead, the activity of the RO requires that it be in its normal position and orientation with respect to the other sequence elements of the rRNA genes. The enhancer-binding transcription factor xUBF also binds to the repetitive sequences of the RO in vitro, but these sequences do not activate transcription in vivo. We propose that the RO is a specialized insulator element that organizes the tandem array of rRNA genes into single-gene expression units by promoting activation of a promoter by its proximal enhancers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.