ArticleMolecular and cellular biology1997
The MEF2A 3' untranslated region functions as a cis-acting translational repressor.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 58 citations in OpenAlex.
- Epitranscriptomics as a New Layer of Regulation of Gene Expression in Skeletal Muscle: Known Functions and Future Perspectives.International journal of molecular sciences · 2023Review
- Myocyte Enhancer Factor 2A Plays a Central Role in the Regulatory Networks of Cellular Physiopathology.Aging and disease · 2023Review
- Review
- Review
- RNA-binding protein AUF1 promotes myogenesis by regulating MEF2C expression levels.Molecular and cellular biology · 2014Article
- miR-92b regulates Mef2 levels through a negative-feedback circuit during Drosophila muscle development.Development (Cambridge, England) · 2012Article
- Differential expression of genes and proteins between electric organ and skeletal muscle in the mormyrid electric fish Brienomyrus brachyistius.The Journal of experimental biology · 2012Article
- Promoter variant-dependent mRNA expression of the MEF2A in longissimus dorsi muscle in cattle.DNA and cell biology · 2012Article
- miR-155 inhibits expression of the MEF2A protein to repress skeletal muscle differentiation.The Journal of biological chemistry · 2011Article
- Nucleotide sequence and variations of the bovine myocyte enhancer factor 2C (MEF2C) gene promoter in Bos taurus cattle.Molecular biology reports · 2011Article
- Unusual conservation among genes encoding small secreted salivary gland proteins from a gall midge.BMC evolutionary biology · 2010Article
- The microRNA miR-1 regulates a MEF-2-dependent retrograde signal at neuromuscular junctions.Cell · 2008Article
- Myocyte enhancer factor 2A is transcriptionally autoregulated.The Journal of biological chemistry · 2008Article
- Phosphorylation and alternative pre-mRNA splicing converge to regulate myocyte enhancer factor 2C activity.Molecular and cellular biology · 2004Article
- HRC is a direct transcriptional target of MEF2 during cardiac, skeletal, and arterial smooth muscle development in vivo.Molecular and cellular biology · 2004Article
- Translational silencing of ceruloplasmin requires the essential elements of mRNA circularization: poly(A) tail, poly(A)-binding protein, and eukaryotic translation initiation factor 4G.Molecular and cellular biology · 2001Article
- Importance of the 3' untranslated region of ornithine decarboxylase mRNA in the translational regulation of the enzyme.The Biochemical journal · 2001Article
- ERK5 is a novel type of mitogen-activated protein kinase containing a transcriptional activation domain.Molecular and cellular biology · 2000Article
- Translation is regulated via the 3' untranslated region of alpha-myosin heavy chain mRNA by calcium but not by its localization.Journal of muscle research and cell motility · 2000Article
- Delayed translational silencing of ceruloplasmin transcript in gamma interferon-activated U937 monocytic cells: role of the 3' untranslated region.Molecular and cellular biology · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myocyte enhancer factor 2 (MEF2) proteins serve as important muscle transcription factors. In addition, MEF2 proteins have been shown to potentiate the activity of other cell-type-specific transcription factors found in muscle and brain tissue. While transcripts for MEF2 factors are widely expressed in a variety of cells and tissues, MEF2 proteins and binding activity are largely restricted to skeletal, smooth, and cardiac muscle and to brain. This disparity between MEF2 protein and mRNA expression suggests that translational control may play an important role in regulating MEF2 expression. In an effort to identify sequences within the MEF2A message which control translation, we isolated the mouse MEF2A 3' untranslated region (UTR) and fused it to the chloramphenicol acetyltransferase (CAT) reporter gene. Here, we show by CAT assay that the MEF2A 3' UTR dramatically inhibits CAT gene expression in vivo and that this inhibition is due to an internal region within the highly conserved 3' UTR. RNase protection analyses demonstrated that the steady-state level of CAT mRNA produced in vivo was not affected by fusion of the MEF2A 3' UTR, indicating that the inhibition of CAT activity resulted from translational repression. Furthermore, fusion of the MEF2A 3' UTR to CAT inhibited translation in vitro in rabbit reticulocyte lysates. We also show that the translational repression mediated by the 3' UTR of MEF2A is regulated during muscle cell differentiation. As muscle cells in culture differentiate, the translational inhibition caused by the MEF2A 3' UTR is relaxed. These results demonstrate that the MEF2A 3' UTR functions as a cis-acting translational repressor both in vitro and in vivo and suggest that this repression may contribute to the tissue-restricted expression and binding activity of MEF2A.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.