ArticleMolecular and cellular biology1997
A specific RNA hairpin loop structure binds the RNA recognition motifs of the Drosophila SR protein B52.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 86 citations in OpenAlex.
- Chromatin Accessibility Reveals Adaptive Regulatory Variation in a Keystone Freshwater Fish.Molecular ecology · 2026Article
- RNA structure in alternative splicing regulation: from mechanism to therapy.Acta biochimica et biophysica Sinica · 2024Review
- Cooperative Binding of SRSF3 to Structured 3'ss-α Exon RNA during α Exon Inclusion in the ZO-1 mRNA.Current issues in molecular biology · 2023Article
- Allele-specific alternative splicing of Drosophila Ribosomal protein S21 suppresses a lethal mutation in the Phosphorylated adaptor for RNA export (Phax) gene.G3 (Bethesda, Md.) · 2022Article
- Secondary structures in RNA synthesis, splicing and translation.Computational and structural biotechnology journal · 2022Review
- Structure of SRSF1 RRM1 bound to RNA reveals an unexpected bimodal mode of interaction and explains its involvement in SMN1 exon7 splicing.Nature communications · 2021Article
- Intrinsic Regulatory Role of RNA Structural Arrangement in Alternative Splicing Control.International journal of molecular sciences · 2020Review
- The SR protein B52/SRp55 regulates splicing of the period thermosensitive intron and mid-day siesta in Drosophila.Scientific reports · 2018Article
- Tethered Function Assays as Tools to Elucidate the Molecular Roles of RNA-Binding Proteins.Advances in experimental medicine and biology · 2016Article
- SR proteins control a complex network of RNA-processing events.RNA (New York, N.Y.) · 2015Article
- New Technologies Provide Quantum Changes in the Scale, Speed, and Success of SELEX Methods and Aptamer Characterization.Molecular therapy. Nucleic acids · 2014Article
- RNA aptamers that functionally interact with green fluorescent protein and its derivatives.Nucleic acids research · 2012Article
- Identification and experimental validation of splicing regulatory elements in Drosophila melanogaster reveals functionally conserved splicing enhancers in metazoans.RNA (New York, N.Y.) · 2011Article
- Commandeering a biological pathway using aptamer-derived molecular adaptors.Nucleic acids research · 2010Article
- Composite RNA aptamers as functional mimics of proteins.Nucleic acids research · 2009Article
- Dissecting the splicing mechanism of the Drosophila editing enzyme; dADAR.Nucleic acids research · 2009Article
- The secondary structure of the human immunodeficiency virus type 1 transcript modulates viral splicing and infectivity.Journal of virology · 2008Article
- TFIIB aptamers inhibit transcription by perturbing PIC formation at distinct stages.Nucleic acids research · 2008Article
- Establishment of a novel in vivo sex-specific splicing assay system to identify a trans-acting factor that negatively regulates splicing of Bombyx mori dsx female exons.Molecular and cellular biology · 2008Article
- A regulator of Dscam mutually exclusive splicing fidelity.Nature structural & molecular biology · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
B52, also known as SRp55, is a member of the Drosophila melanogaster SR protein family, a group of nuclear proteins that are both essential splicing factors and specific splicing regulators. Like most SR proteins, B52 contains two RNA recognition motifs in the N terminus and a C-terminal domain rich in serine-arginine dipeptide repeats. Since B52 is an essential protein and is expected to play a role in splicing a subset of Drosophila pre-mRNAs, its function is likely to be mediated by specific interactions with RNA. To investigate the RNA-binding specificity of B52, we isolated B52-binding RNAs by selection and amplification from a pool of random RNA sequences by using full-length B52 protein as the target. These RNAs contained a conserved consensus motif that constitutes the core of a secondary structural element predicted by energy minimization. Deletion and substitution mutations defined the B52-binding site on these RNAs as a hairpin loop structure covering about 20 nucleotides, which was confirmed by structure-specific enzymatic probing. Finally, we demonstrated that both RNA recognition motifs of B52 are required for RNA binding, while the RS domain is not involved in this interaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.