ArticleProceedings of the National Academy of Sciences of the United States of America1997
Regulated bidirectional motility of melanophore pigment granules along microtubules in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 223 citations in OpenAlex.
- Article
- Systems mapping of bidirectional endosomal transport through the crowded cell.Current biology : CB · 2024Article
- The physical and cellular mechanism of structural color change in zebrafish.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Reconstitution of Neuronal Motor Traffic on Septin-Associated Microtubules.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks.Scientific reports · 2023Article
- Phosphoregulation of Kinesins Involved in Long-Range Intracellular Transport.Frontiers in cell and developmental biology · 2022Review
- A role for Dynlt3 in melanosome movement, distribution, acidity and transfer.Communications biology · 2021Article
- Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C.The Journal of cell biology · 2019Article
- Load-dependent detachment kinetics plays a key role in bidirectional cargo transport by kinesin and dynein.Traffic (Copenhagen, Denmark) · 2019Article
- Crowd Control: Effects of Physical Crowding on Cargo Movement in Healthy and Diseased Neurons.Frontiers in cellular neuroscience · 2019Review
- Retraction of rod-like mitochondria during microtubule-dependent transport.Bioscience reports · 2018Article
- Characterization of microtubule buckling in living cells.European biophysics journal : EBJ · 2017Article
- Myosin Va's adaptor protein melanophilin enforces track selection on the microtubule and actin networks in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Kinesin-2 KIF3AB exhibits novel ATPase characteristics.The Journal of biological chemistry · 2014Article
- Motor coupling through lipid membranes enhances transport velocities for ensembles of myosin Va.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Bidirectional cargo transport: moving beyond tug of war.Nature reviews. Molecular cell biology · 2014Review
- Lateral motion and bending of microtubules studied with a new single-filament tracking routine in living cells.Biophysical journal · 2014Article
- Single-molecule fluorescence and in vivo optical traps: how multiple dyneins and kinesins interact.Chemical reviews · 2014Review
- Reconstituting the motility of isolated intracellular cargoes.Methods in enzymology · 2014Article
- Lipid droplets purified from Drosophila embryos as an endogenous handle for precise motor transport measurements.Biophysical journal · 2013Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although many types of membrane-bound organelles rely upon microtubule-based transport for their proper placement within the cytoplasm, the molecular mechanisms that regulate intracellular motility remain largely unknown. To address this problem, we have studied the microtubule-dependent dispersion and aggregation of pigment granules from an immortalized Xenopus melanophore cell line. We have reconstituted pigment granule motility along bovine brain microtubules in vitro using a microscope-based motility assay. Pigment granules, or melanosomes, move along single microtubules bidirectionally; however, analysis of the polarities of this movement shows that melanosomes that have been purified from dispersed cells exhibit mostly plus end-directed motility, while movement of organelles from aggregating cells is biased toward the minus end. Removal of all soluble proteins from the melanosome fractions by density gradient centrifugation does not diminish organelle motility, demonstrating that all the components required for transport have a stable association with the melanosome membranes. Western blotting shows the presence of the plus end-directed motor, kinesin-II, and the minus end-directed motor, cytoplasmic dynein in highly purified melanosomes. Therefore, purified melanosomes retain their ability to move along microtubules as well as their regulated state. Direct biochemical comparison of melanosomes from aggregated and dispersed cells may elucidate the molecular mechanisms that regulate organelle transport in melanophores.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.