ArticleProceedings of the National Academy of Sciences of the United States of America1997
RNA splicing specificity determined by the coordinated action of RNA recognition motifs in SR proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 103 citations in OpenAlex.
- Towards understandings of serine/arginine-rich splicing factors.Acta pharmaceutica Sinica. B · 2023Review
- Cooperative engagement and subsequent selective displacement of SR proteins define the pre-mRNA 3D structural scaffold for early spliceosome assembly.Nucleic acids research · 2022Article
- SRSF6 Regulates the Alternative Splicing of the Apoptotic Fas Gene by Targeting a Novel RNA Sequence.Cancers · 2022Article
- Exon-independent recruitment of SRSF1 is mediated by U1 snRNP stem-loop 3.The EMBO journal · 2022Article
- Poison Exon Splicing Regulates a Coordinated Network of SR Protein Expression during Differentiation and Tumorigenesis.Molecular cell · 2020Article
- Structural disruption of exonic stem-loops immediately upstream of the intron regulates mammalian splicing.Nucleic acids research · 2020Article
- PRMT5 methylome profiling uncovers a direct link to splicing regulation in acute myeloid leukemia.Nature structural & molecular biology · 2019Article
- A U2-snRNP-independent role of SF3b in promoting mRNA export.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- SRSF2 mutations drive oncogenesis by activating a global program of aberrant alternative splicing in hematopoietic cells.Leukemia · 2018Article
- Intronless mRNAs transit through nuclear speckles to gain export competence.The Journal of cell biology · 2018Article
- SR proteins regulate V6 exon splicing of CD44 pre-mRNA.BMB reports · 2016Article
- The exosome controls alternative splicing by mediating the gene expression and assembly of the spliceosome complex.Scientific reports · 2015Article
- SRSF1 RNA Recognition Motifs Are Strong Inhibitors of HIV-1 Replication.Journal of virology · 2015Article
- PKC-Theta is a Novel SC35 Splicing Factor Regulator in Response to T Cell Activation.Frontiers in immunology · 2015Article
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- Isolated pseudo-RNA-recognition motifs of SR proteins can regulate splicing using a noncanonical mode of RNA recognition.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
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- CDK13, a new potential human immunodeficiency virus type 1 inhibitory factor regulating viral mRNA splicing.Journal of virology · 2008Article
- The serine/arginine-rich protein family in rice plays important roles in constitutive and alternative splicing of pre-mRNA.The Plant cell · 2006Article
- Small molecule control of pre-mRNA splicing.RNA (New York, N.Y.) · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pre-mRNA splicing requires a large number of RNA-binding proteins that have one or more RNA-recognition motifs (RRMs). Among these is the SR protein family, whose members are essential for splicing and are able to commit pre-mRNAs to the splicing pathway with overlapping but distinct substrate specificity. Some SR proteins, such as SC35, contain an N-terminal RRM and a C-terminal arginine/serine-rich (RS) domain, whereas others, such as SF2/ASF, also contain a second, atypical RRM. Although both the RRMs and the RS domain of SR proteins are required for constitutive splicing, it is unclear which domain(s) defines their substrate specificity, and whether two RRMs in a given SR protein function independently or act coordinately. Using domain swaps between SC35 and SF2/ASF and a functional commitment assay, we demonstrate that individual domains are functional modules, RS domains are interchangeable, and substrate specificity is defined by the RRMs. The atypical RRM of SF2/ASF does not appear to function alone in splicing, but can either activate or suppress the splicing specificity of an N-terminal RRM. Therefore, multiple RRMs in SR proteins act coordinately to achieve a unique spectrum of pre-mRNA substrate specificity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.