ArticleThe Journal of cell biology1997
Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of alpha/beta tubulin.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 97 citations in OpenAlex.
- The amorphous pericentriolar cloud takes shape.Nature cell biology · 2012Article
- The small organic compound HMN-176 delays satisfaction of the spindle assembly checkpoint by inhibiting centrosome-dependent microtubule nucleation.Molecular cancer therapeutics · 2009Article
- Centrosome-associated RNA in surf clam oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Structure and microtubule-nucleation activity of isolated Drosophila embryo centrosomes characterized by whole mount scanning and transmission electron microscopy.Histochemistry and cell biology · 2005Article
- The kinetically dominant assembly pathway for centrosomal asters in Caenorhabditis elegans is gamma-tubulin dependent.The Journal of cell biology · 2002Article
- Reconstitution and characterization of budding yeast gamma-tubulin complex.Molecular biology of the cell · 2002Article
- Characterization of Cep135, a novel coiled-coil centrosomal protein involved in microtubule organization in mammalian cells.The Journal of cell biology · 2002Article
- GCP5 and GCP6: two new members of the human gamma-tubulin complex.Molecular biology of the cell · 2001Article
- Outer dense fiber 2 is a widespread centrosome scaffold component preferentially associated with mother centrioles: its identification from isolated centrosomes.Molecular biology of the cell · 2001Article
- Identification of ribonucleotide reductase protein R1 as an activator of microtubule nucleation in Xenopus egg mitotic extracts.Molecular biology of the cell · 2000Article
- Nek2B, a novel maternal form of Nek2 kinase, is essential for the assembly or maintenance of centrosomes in early Xenopus embryos.The EMBO journal · 2000Article
- Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubules.The Journal of cell biology · 1999Article
- Differential regulation of maternal vs. paternal centrosomes.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Recruitment of the gamma-tubulin ring complex to Drosophila salt-stripped centrosome scaffolds.The Journal of cell biology · 1998Article
- The disassembly and reassembly of functional centrosomes in vitro.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The mammalian gamma-tubulin complex contains homologues of the yeast spindle pole body components spc97p and spc98p.The Journal of cell biology · 1998Article
- Xgrip109: a gamma tubulin-associated protein with an essential role in gamma tubulin ring complex (gammaTuRC) assembly and centrosome function.The Journal of cell biology · 1998Article
- Pericentrin and gamma-tubulin form a protein complex and are organized into a novel lattice at the centrosome.The Journal of cell biology · 1998Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Centrosome-dependent microtubule nucleation involves the interaction of tubulin subunits with pericentriolar material. To study the biochemical and structural basis of centrosome-dependent microtubule nucleation, centrosomes capable of organizing microtubules into astral arrays were isolated from parthenogenetically activated Spisula solidissima oocytes. Intermediate voltage electron microscopy tomography revealed that each centrosome was composed of a single centriole surrounded by pericentriolar material that was studded with ring-shaped structures approximately 25 nm in diameter and <25 nm in length. A number of proteins copurified with centrosomes including: (a) proteins that contained M-phase-specific phosphoepitopes (MPM-2), (b) alpha-, beta-, and gamma-tubulins, (c) actin, and (d) three low molecular weight proteins of <20 kD. gamma-Tubulin was not an MPM-2 phosphoprotein and was the most abundant form of tubulin in centrosomes. Relatively little alpha- or beta-tubulin copurified with centrosomes, and the ratio of alpha- to beta-tubulin in centrosomes was not 1:1 as expected, but rather 1:4.6, suggesting that centrosomes contain beta-tubulin that is not dimerized with alpha-tubulin.
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Registered trials
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