ArticleThe Journal of cell biology1997
cdc12p, a protein required for cytokinesis in fission yeast, is a component of the cell division ring and interacts with profilin.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 176 papers.
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176 citing papers in PubMed, 411 citations in OpenAlex.
- Leiomodin 2 is a processive pointed-end elongator of actin filaments.Nature communications · 2026Article
- Leiomodin 2 functions as a processive pointed-end elongator of actin filaments.bioRxiv : the preprint server for biology · 2026Article
- Regulation of Yeast Cytokinesis by Calcium.Journal of fungi (Basel, Switzerland) · 2025Review
- FHOD-1 and profilin protect sarcomeres against contraction-induced deformation> inMolecular biology of the cell · 2024Article
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- Actin assembly requirements of the formin Fus1 to build the fusion focus.Journal of cell science · 2022Article
- Cdk1 phosphorylation of fission yeast paxillin inhibits its cytokinetic ring localization.Molecular biology of the cell · 2021Article
- Formin Cdc12's specific actin assembly properties are tailored for cytokinesis in fission yeast.Biophysical journal · 2021Article
- Time-varying mobility and turnover of actomyosin ring components during cytokinesis inMolecular biology of the cell · 2021Article
- Escape from mitotic catastrophe by actin-dependent nuclear displacement in fission yeast.iScience · 2021Article
- Calcium spikes accompany cleavage furrow ingression and cell separation during fission yeast cytokinesis.Molecular biology of the cell · 2021Article
- Profilin's Affinity for Formin Regulates the Availability of Filament Ends for Actin Monomer Binding.Journal of molecular biology · 2020Article
- A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast.eLife · 2020Article
- FHOD-1 is the only formin in Caenorhabditis elegans that promotes striated muscle growth and Z-line organization in a cell autonomous manner.Cytoskeleton (Hoboken, N.J.) · 2020Article
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- Genetic suppression of defective profilin by attenuated Myosin II reveals a potential role for Myosin II in actin dynamics in vivo in fission yeast.Molecular biology of the cell · 2020Article
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- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
- TwoThe Journal of cell biology · 2019Article
- Fission yeast TRP channel Pkd2p localizes to the cleavage furrow and regulates cell separation during cytokinesis.Molecular biology of the cell · 2019Article
116 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As in many other eukaryotic cells, cell division in fission yeast depends on the assembly of an actin ring that circumscribes the middle of the cell. Schizosaccharomyces pombe cdc12 is an essential gene necessary for actin ring assembly and septum formation. Here we show that cdc12p is a member of a family of proteins including Drosophila diaphanous, Saccharomyces cerevisiae BNI1, and S. pombe fus1, which are involved in cytokinesis or other actin-mediated processes. Using indirect immunofluorescence, we show that cdc12p is located in the cell division ring and not in other actin structures. When overexpressed, cdc12p is located at a medial spot in interphase that anticipates the future ring site. cdc12p localization is altered in actin ring mutants. cdc8 (tropomyosin homologue), cdc3 (profilin homologue), and cdc15 mutants exhibit no specific cdc12p staining during mitosis. cdc4 mutant cells exhibit a medial cortical cdc12p spot in place of a ring. mid1 mutant cells generally exhibit a cdc12p spot with a single cdc12p strand extending in a random direction. Based on these patterns, we present a model in which ring assembly originates from a single point on the cortex and in which a molecular pathway for the functions of cytokinesis proteins is suggested. Finally, we found that cdc12 and cdc3 mutants show a synthetic-lethal genetic interaction, and a proline-rich domain of cdc12p binds directly to profilin cdc3p in vitro, suggesting that one function of cdc12p in ring assembly is to bind profilin.
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