ArticleThe Journal of cell biology1997
Molecular requirements for bi-directional movement of phagosomes along microtubules.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
78 citing papers in PubMed, 232 citations in OpenAlex.
- Huntingtin polyglutamine expansions misdirect axonal transport by perturbing motor and adaptor recruitment.iScience · 2026Article
- Many dynein teams collectively generate high forces during the transport of large organelles.Biophysical journal · 2026Article
- Phagocytosis at a glance.Journal of cell science · 2025Review
- Phosphatidic acid-dependent recruitment of microtubule motors to spherical supported lipid bilayers for in vitro motility assays.Cell reports · 2024Article
- Ninein promotes F-actin cup formation and inward phagosome movement during phagocytosis in macrophages.Molecular biology of the cell · 2024Article
- Timing of Phagosome Maturation Depends on Their Transport Switching from Actin to Microtubule Tracks.The journal of physical chemistry. B · 2023Article
- Huntingtin S421 phosphorylation increases kinesin and dynein engagement on early endosomes and lysosomes.Biophysical journal · 2023Article
- The spatial resolution limit of phagocytosis.Biophysical journal · 2023Article
- StARD9 is a novel lysosomal kinesin required for membrane tubulation, cholesterol transport and Purkinje cell survival.Journal of cell science · 2023Article
- BORC complex specific components and Kinesin-1 mediate autophagy evasion by the autophagy-resistant Mycobacterium tuberculosis Beijing strain.Scientific reports · 2023Article
- Kinetics of phagosome maturation is coupled to their intracellular motility.Communications biology · 2022Article
- A guide to measuring phagosomal dynamics.The FEBS journal · 2021Review
- Motor Proteins and Spermatogenesis.Advances in experimental medicine and biology · 2021Review
- Squeezing in a Meal: Myosin Functions in Phagocytosis.Trends in cell biology · 2020Review
- A guided approach for subtomogram averaging of challenging macromolecular assemblies.Journal of structural biology: X · 2020Article
- Modulation of Immune Responses by Particle Size and Shape.Frontiers in immunology · 2020Review
- Setting the dynein motor in motion: New insights from electron tomography.The Journal of biological chemistry · 2019Review
- Tau directs intracellular trafficking by regulating the forces exerted by kinesin and dynein teams.Traffic (Copenhagen, Denmark) · 2018Article
- Fibronectin (FN) cooperated with TLR2/TLR4 receptor to promote innate immune responses of macrophages via binding to integrin β1.Virulence · 2018Article
- Defective Phagocytic Properties of HIV-Infected Macrophages: How Might They Be Implicated in the Development of InvasiveFrontiers in immunology · 2018Review
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Microtubules facilitate the maturation of phagosomes by favoring their interactions with endocytic compartments. Here, we show that phagosomes move within cells along tracks of several microns centrifugally and centripetally in a pH- and microtubule-dependent manner. Phagosome movement was reconstituted in vitro and required energy, cytosol and membrane proteins of this organelle. The activity or presence of these phagosome proteins was regulated as the organelle matured, with "late" phagosomes moving threefold more frequently than "early" ones. The majority of moving phagosomes were minus-end directed; the remainder moved towards microtubule plus-ends and a small subset moved bi-directionally. Minus-end movement showed pharmacological characteristics expected for dyneins, was inhibited by immunodepletion of cytoplasmic dynein and could be restored by addition of cytoplasmic dynein. Plus-end movement displayed pharmacological properties of kinesin, was inhibited partially by immunodepletion of kinesin and fully by addition of an anti-kinesin IgG. Immunodepletion of dynactin, a dynein-activating complex, inhibited only minus-end directed motility. Evidence is provided for a dynactin-associated kinase required for dynein-mediated vesicle transport. Movement in both directions was inhibited by peptide fragments from kinectin (a putative kinesin membrane receptor), derived from the region to which a motility-blocking antibody binds. Polypeptide subunits from these microtubule-based motility factors were detected on phagosomes by immunoblotting or immunoelectron microscopy. This is the first study using a single in vitro system that describes the roles played by kinesin, kinectin, cytoplasmic dynein, and dynactin in the microtubule-mediated movement of a purified membrane organelle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.