ArticleJournal of bacteriology1997
Identification, cloning, and characterization of the Ima operon, whose gene products are unique to Listeria monocytogenes.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Functional Genomics Identified Novel Genes Involved in Growth at Low Temperatures in Listeria monocytogenes.Microbiology spectrum · 2022Article
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- An Effective Counterselection System for Listeria monocytogenes and Its Use To Characterize the Monocin Genomic Region of Strain 10403S.Applied and environmental microbiology · 2017Article
- Analysis of theFrontiers in microbiology · 2017Article
- F-Type Bacteriocins of Listeria monocytogenes: a New Class of Phage Tail-Like Structures Reveals Broad Parallel Coevolution between Tailed Bacteriophages and High-Molecular-Weight Bacteriocins.Journal of bacteriology · 2016Article
- Comparative Genomics of theFrontiers in microbiology · 2016Article
- Sublethal Concentrations of Antibiotics Cause Shift to Anaerobic Metabolism in Listeria monocytogenes and Induce Phenotypes Linked to Antibiotic Tolerance.Frontiers in microbiology · 2016Article
- Article
- Review
- Reassessment of the Listeria monocytogenes pan-genome reveals dynamic integration hotspots and mobile genetic elements as major components of the accessory genome.BMC genomics · 2013Article
- Molecular approaches to the identification of pathogenic and nonpathogenic listeriae.Microbiology insights · 2013Review
- Listeria monocytogenes grown at 7° C shows reduced acid survival and an altered transcriptional response to acid shock compared to L. monocytogenes grown at 37° C.Applied and environmental microbiology · 2012Article
- Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes.BMC genomics · 2012Article
- Identification of components of the sigma B regulon in Listeria monocytogenes that contribute to acid and salt tolerance.Applied and environmental microbiology · 2008Article
- Transcriptomic and phenotypic analyses suggest a network between the transcriptional regulators HrcA and sigmaB in Listeria monocytogenes.Applied and environmental microbiology · 2007Article
- Microarray-based characterization of the Listeria monocytogenes cold regulon in log- and stationary-phase cells.Applied and environmental microbiology · 2007Article
- Natural atypical Listeria innocua strains with Listeria monocytogenes pathogenicity island 1 genes.Applied and environmental microbiology · 2004Article
- Genetic analysis of a bacterial genetic exchange element: the gene transfer agent of Rhodobacter capsulatus.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lmaA gene of Listeria monocytogenes encodes a protein capable of inducing delayed-type hypersensitivity reactions in L. monocytogenes-immune mice (S. Göhmann, M. Leimeister-Wachter, E. Schiltz, W. Goebel, and T. Chakraborty, M. Microbiol. 4:1091-1099, 1990). Here we show that it is the last gene of the lma operon, which now comprises four genes, lmaDCBA. Maxicell analysis of peptides encoded by the lma operon identified four polypeptides of 16.7, 16.4, 14.9, and 21 kDa which correspond to the gene products encoded by the lmaD, -C, -B, and -A genes, respectively. Northern blot analysis of the lma operon showed that lmaA is expressed by two transcripts: the longer lmaDCBA transcript of 2,100 nucleotides, which was observed at growth temperatures of 37 and 20 degrees C, and a shorter transcript consisting of lmaBA, which is detected only at low temperatures (20 degrees C). Two promoters, one preceding the lmaD gene and another located upstream of the lmaB gene, were detected. An extended stem-loop structure resembling box elements found in other gram-positive pathogens was also present in the lmaC-lmaB intergenic region. By immunoblot analysis, we found that although LmaA was produced at both temperatures (20 and 37 degrees C), it was secreted into culture supernatants only at 20 degrees C. However, LmaA lacks a bona fide signal peptide sequence and could, like flagellin, be secreted by a type III transport system. DNA hybridization studies indicate that the lma operon is species specific and restricted to pathogenic strains of L. monocytogenes.
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