ArticleJournal of bacteriology1997
Minimal Streptomyces sp. strain C5 daunorubicin polyketide biosynthesis genes required for aklanonic acid biosynthesis.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 71 citations in OpenAlex.
- A BioBricks Metabolic Engineering Platform for the Biosynthesis of Anthracyclinones inACS synthetic biology · 2022Article
- Genome mining of biosynthetic and chemotherapeutic gene clusters in Streptomyces bacteria.Scientific reports · 2020Article
- Biosynthesis of Polyketides inMicroorganisms · 2019Review
- Structural and functional analysis of two di-domain aromatase/cyclases from type II polyketide synthases.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cloning and sequencing of the kedarcidin biosynthetic gene cluster from Streptoalloteichus sp. ATCC 53650 revealing new insights into biosynthesis of the enediyne family of antitumor antibiotics.Molecular bioSystems · 2013Article
- Biosynthetic mechanism for sunscreens of the biocontrol agent Lysobacter enzymogenes.PloS one · 2013Article
- Metagenomic analysis of Streptomyces lividans reveals host-dependent functional expression.Applied and environmental microbiology · 2012Article
- Identification and characterization of the anti-methicillin-resistant Staphylococcus aureus WAP-8294A2 biosynthetic gene cluster from Lysobacter enzymogenes OH11.Antimicrobial agents and chemotherapy · 2011Article
- The biosynthetic gene cluster of zorbamycin, a member of the bleomycin family of antitumor antibiotics, from Streptomyces flavoviridis ATCC 21892.Molecular bioSystems · 2009Article
- Structure and biosynthesis of heat-stable antifungal factor (HSAF), a broad-spectrum antimycotic with a novel mode of action.Antimicrobial agents and chemotherapy · 2007Article
- Characterization of the Streptomyces sp. strain C5 snp locus and development of snp-derived expression vectors.Applied and environmental microbiology · 2003Article
- Identification of a cyclase gene dictating the C-9 stereochemistry of anthracyclines from Streptomyces nogalater.Antimicrobial agents and chemotherapy · 2000Article
- A multiplasmid approach to preparing large libraries of polyketides.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- The Streptomyces peucetius dpsC gene determines the choice of starter unit in biosynthesis of the daunorubicin polyketide.Journal of bacteriology · 1999Article
- Nonactin biosynthesis: the product of nonS catalyzes the formation of the furan ring of nonactic acid.Antimicrobial agents and chemotherapy · 1999Article
- Doxorubicin overproduction in Streptomyces peucetius: cloning and characterization of the dnrU ketoreductase and dnrV genes and the doxA cytochrome P-450 hydroxylase gene.Journal of bacteriology · 1999Article
- The Streptomyces peucetius dpsY and dnrX genes govern early and late steps of daunorubicin and doxorubicin biosynthesis.Journal of bacteriology · 1998Article
- A study of iterative type II polyketide synthases, using bacterial genes cloned from soil DNA: a means to access and use genes from uncultured microorganisms.Journal of bacteriology · 1997Article
- Cloning of an avilamycin biosynthetic gene cluster from Streptomyces viridochromogenes Tü57.Journal of bacteriology · 1997Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The structure of the Streptomyces sp. strain C5 daunorubicin type II polyketide synthase (PKS) gene region is different from that of other known type II PKS gene clusters. Directly downstream of the genes encoding ketoacylsynthase alpha and beta (KS alpha, KS beta) are two genes (dpsC, dpsD) encoding proteins of unproven function, both absent from other type II PKS gene clusters. Also in contrast to other type II PKS clusters, the gene encoding the acyl carrier protein (ACP), dpsG, is located about 6.8 kbp upstream of the genes encoding the daunorubicin KS alpha and KS beta. In this work, we demonstrate that the minimal genes required to produce aklanonic acid in heterologous hosts are dpsG (ACP), dauI (regulatory activator), dpsA (KS alpha), dpsB (KS beta), dpsF (aromatase), dpsE (polyketide reductase), and dauG (putative deoxyaklanonic acid oxygenase). The two unusual open reading frames, dpsC (KASIII homolog lacking a known active site) and dpsD (acyltransferase homolog), are not required to synthesize aklanonic acid. Additionally, replacement of dpsD or dpsCD in Streptomyces sp. strain C5 with a neomycin resistance gene (aphI) results in mutant strains that still produced anthracyclines.
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