ArticleApplied and environmental microbiology1997
Infection of Acanthamoeba castellanii by Chlamydia pneumoniae.
Article in Applied and environmental microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed.
- Acanthamoeba spp.: Neglected Protists in Veterinary Medicine.Acta parasitologica · 2025Review
- Molecular identification, phylogenetic analysis and histopathological study of pathogenic free-living amoebae isolated from discus fish (Symphysodon aequifasciatus) in Iran: 2020-2022.BMC veterinary research · 2024Article
- Temperature Affects the Host Range of Rhabdochlamydia porcellionis.Applied and environmental microbiology · 2023Article
- Microbial warfare in the wild-the impact of protists on the evolution and virulence of bacterial pathogens.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- War of the microbial world: Acanthamoeba spp. interactions with microorganisms.Folia microbiologica · 2021Review
- Differentially Expressed Gene Profile of Acanthamoeba castellanii Induced by an Endosymbiont Legionella pneumophila.The Korean journal of parasitology · 2021Article
- Free-Living Amoebae as Hosts for and Vectors of Intracellular Microorganisms with Public Health Significance.Viruses · 2017Review
- Optimized methods for Legionella pneumophila release from its Acanthamoeba hosts.BMC microbiology · 2016Article
- Assessment of Chlamydia psittaci Shedding and Environmental Contamination as Potential Sources of Worker Exposure throughout the Mule Duck Breeding Process.Applied and environmental microbiology · 2015Article
- HACCP-Based Programs for Preventing Disease and Injury from Premise Plumbing: A Building Consensus.Pathogens (Basel, Switzerland) · 2015Review
- Current and past strategies for bacterial culture in clinical microbiology.Clinical microbiology reviews · 2015Review
- Relevance of free-living amoebae as hosts for phylogenetically diverse microorganisms.Parasitology research · 2014Review
- Potential role of bacteria packaging by protozoa in the persistence and transmission of pathogenic bacteria.Frontiers in microbiology · 2014Article
- Salmonella transcriptional signature in Tetrahymena phagosomes and role of acid tolerance in passage through the protist.The ISME journal · 2011Article
- Legionella, protozoa, and biofilms: interactions within complex microbial systems.Microbial ecology · 2009Review
- Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients.The Korean journal of parasitology · 2007Article
- Pathogenic potential of novel Chlamydiae and diagnostic approaches to infections due to these obligate intracellular bacteria.Clinical microbiology reviews · 2006Review
- Interaction of Pasteurella multocida with free-living amoebae.Applied and environmental microbiology · 2005Article
- Chlamydophila pneumoniae antibodies in office workers with and without inflammatory rheumatic diseases in a moisture-damaged building.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2005Article
- Isolation of Waddlia malaysiensis, a novel intracellular bacterium, from fruit bat (Eonycteris spelaea).Emerging infectious diseases · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydia pneumoniae is an intracellular respiratory pathogen, which, similar to Legionella, might have developed mechanisms to escape the intracellular bactericidal activity of both human host cells and amoeba. We therefore investigated the intracellular growth and survival of C. pneumoniae in Acanthamoeba castellanii by using cell culture, immunofluorescence microscopy, and electron microscopy. A castellanii was incubated with purified elementary bodies of C. pneumoniae TW 183 at a concentration of 10(6) inclusion-forming units (IFU)/ml to give a ratio of approximately 1 IFU of C. pneumoniae per amoeba. Quantitative determination of chlamydial growth within A. castellanii revealed viable and infective C. pneumoniae in the range of 10(4) to 10(5) IFU/ml between days 7 and 14 postinfection. Immunofluorescence analysis and transmission electron microscopy with subsequent immunogold staining confirmed evidence of infection of the amoebae by C. Pneumoniae and additionally revealed that C. pneumoniae entered the typical growth cycle. Our results show that amoebae allow the survival of C. pneumoniae, suggesting that amoebae may serve as an additional reservoir for Chlamydia or Chlamydia-related organisms.
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