ArticleApplied and environmental microbiology1997
Construction of a starch-utilizing yeast by cell surface engineering.
Article in Applied and environmental microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 166 citations in OpenAlex.
- Article
- Progress of Molecular Display Technology UsingMicroorganisms · 2023Review
- Fungal biotechnology: From yesterday to tomorrow.Frontiers in fungal biology · 2023Review
- Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Development of a yeast cell surface display method using the SpyTag/SpyCatcher system.Scientific reports · 2021Article
- Engineering Escherichia coli for Direct Production of 1,2-Propanediol and 1,3-Propanediol from Starch.Current microbiology · 2020Article
- Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody.Antibodies (Basel, Switzerland) · 2017Article
- Advances in the Isolation of Specific Monoclonal Rabbit Antibodies.Frontiers in immunology · 2017Review
- Rapid preparation of mutated influenza hemagglutinins for influenza virus pandemic prevention.AMB Express · 2016Article
- Construction of a highly active xylanase displaying oleaginous yeast: comparison of anchoring systems.PloS one · 2014Article
- Direct production of organic acids from starch by cell surface-engineered Corynebacterium glutamicum in anaerobic conditions.AMB Express · 2013Article
- Arming Technology in Yeast-Novel Strategy for Whole-cell Biocatalyst and Protein Engineering.Biomolecules · 2013Article
- Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases.FEBS open bio · 2013Article
- Molecular breeding of advanced microorganisms for biofuel production.Journal of biomedicine & biotechnology · 2011Article
- Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module.Applied microbiology and biotechnology · 2010Article
- Surface displayed expression of a neutralizing epitope of spike protein from a Korean strain of porcine epidemic diarrhea virus.Biotechnology and bioprocess engineering : BBE · 2007Article
- Construction of a library of human glycosyltransferases immobilized in the cell wall of Saccharomyces cerevisiae.Applied and environmental microbiology · 2006Article
- Enhanced reactivity of Rhizopus oryzae lipase displayed on yeast cell surfaces in organic solvents: potential as a whole-cell biocatalyst in organic solvents.Applied and environmental microbiology · 2005Article
- Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and alpha-amylase.Applied and environmental microbiology · 2004Article
- Expression of human glycosyltransferase genes in yeast as a tool for enzymatic synthesis of sugar chain.Glycoconjugate journal · 2004Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have engineered the cell surface of the yeast Saccharomyces cerevisiae by anchoring active glucoamylase protein on the cell wall, and we have endowed the yeast cells with the ability to utilize starch directly as the sole carbon source. The gene encoding Rhizopus oryzae glucoamylase with its secretion signal peptide was fused with the gene encoding the C-terminal half (320 amino acid residues from the C terminus) of yeast alpha-agglutinin, a protein involved in mating and covalently anchored to the cell wall. The constructed plasmid containing this fusion gene was introduced into S. cerevisiae and expressed under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter from S. cerevisiae. The glucoamylase activity as not detected in the culture medium, but it was detected in the cell pellet fraction. The glucoamylase protein transferred to the soluble fraction from the cell wall fraction after glucanase treatment but not after sodium dodecyl sulfate treatment, indicating the covalent binding of the fusion protein to the cell wall. Display of the fused protein was further confirmed by immunofluorescence microscopy and immunoelectron microscopy. The transformant cells could surely grow on starch as the sole carbon source. These results showed that the glucoamylase was anchored on the cell wall and displayed as its active form. This is the first example of an application of cell surface engineering to utilize and improve the metabolic ability of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.