ArticleProceedings of the National Academy of Sciences of the United States of America1997
Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 51 citations in OpenAlex.
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- Chimeric green fluorescent protein-aequorin as bioluminescent Ca2+ reporters at the single-cell level.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Mucosal mast cell secretion processes imaged using three-photon microscopy of 5-hydroxytryptamine autofluorescence.Biophysical journal · 1999Article
- Evanescent-wave microscopy: a new tool to gain insight into the control of transmitter release.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 1999Review
- A vital stain for studying membrane dynamics in bacteria: a novel mechanism controlling septation during Bacillus subtilis sporulation.Molecular microbiology · 1999Article
- Construction of hybrid proteins that migrate retrogradely and transynaptically into the central nervous system.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emission of light, coupled to exocytosis, can in principle be utilized to monitor the activity of a large number of individual synapses simultaneously. To illustrate this concept, fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP-2/synaptobrevin (which we term "synaptolucins") were expressed in cultured hippocampal neurons with the help of viral vectors. Synaptolucins were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with their cognate luciferin, which was added to the extracellular medium. Photon emissions required a depolarizing stimulus, occurred from regions with high synaptic density as ascertained by vital staining of recycling synaptic vesicles, and were sensitive to Ca2+ depletion and clostridial neurotoxins. The method can currently detect exocytosis of the readily releasable pool of synaptic vesicles at a hippocampal synapse, corresponding to about two dozen quanta, but has the potential for greater sensitivity.
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