ArticleProceedings of the National Academy of Sciences of the United States of America1997
Functional antagonism between RNA polymerase II holoenzyme and global negative regulator NC2 in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 62 citations in OpenAlex.
- Structures and implications of TBP-nucleosome complexes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Analyzing the Dynamics of Single TBP-DNA-NC2 Complexes Using Hidden Markov Models.Biophysical journal · 2018Article
- Article
- Defects in the NC2 repressor affect both canonical and non-coding RNA polymerase II transcription initiation in yeast.BMC genomics · 2016Article
- Ncb2 is involved in activated transcription of CDR1 in azole-resistant clinical isolates of Candida albicans.Eukaryotic cell · 2011Article
- Dr1 (NC2) is present at tRNA genes and represses their transcription in human cells.Nucleic acids research · 2010Article
- The yeast RNA polymerase II-associated factor Iwr1p is involved in the basal and regulated transcription of specific genes.The Journal of biological chemistry · 2009Article
- Regulation of nuclear import and export of negative cofactor 2.The Journal of biological chemistry · 2009Article
- Global distribution of negative cofactor 2 subunit-alpha on human promoters.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Article
- The mediator of RNA polymerase II.Chromosoma · 2005Review
- An early function during transcription for the yeast mRNA export factor Dbp5p/Rat8p suggested by its genetic and physical interactions with transcription factor IIH components.Molecular biology of the cell · 2003Article
- The NC2 alpha and beta subunits play different roles in vivo.Genes & development · 2002Article
- Mot1 associates with transcriptionally active promoters and inhibits association of NC2 in Saccharomyces cerevisiae.Molecular and cellular biology · 2002Article
- Direct stimulation of transcription by negative cofactor 2 (NC2) through TATA-binding protein (TBP).Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Functional dissection of a Rice Dr1/DrAp1 transcriptional repression complex.The Plant cell · 2002Article
- Multiple functions of the nonconserved N-terminal domain of yeast TATA-binding protein.Genetics · 2001Article
- Yeast NC2 associates with the RNA polymerase II preinitiation complex and selectively affects transcription in vivo.Molecular and cellular biology · 2001Article
- The C-terminal domain-phosphorylated IIO form of RNA polymerase II is associated with the transcription repressor NC2 (Dr1/DRAP1) and is required for transcription activation in human nuclear extracts.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Genetic analysis of the YDR1-BUR6 repressor complex reveals an intricate balance among transcriptional regulatory proteins in yeast.Molecular and cellular biology · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activation of eukaryotic class II gene expression involves the formation of a transcription initiation complex that includes RNA polymerase II, general transcription factors, and SRB components of the holoenzyme. Negative regulators of transcription have been described, but it is not clear whether any are general repressors of class II genes in vivo. We reasoned that defects in truly global negative regulators should compensate for deficiencies in SRB4 because SRB4 plays a positive role in holoenzyme function. Genetic experiments reveal that this is indeed the case: a defect in the yeast homologue of the human negative regulator NC2 (Dr1 x DRAP1) suppresses a mutation in SRB4. Global defects in mRNA synthesis caused by the defective yeast holoenzyme are alleviated by the NC2 suppressing mutation in vivo, indicating that yeast NC2 is a global negative regulator of class II transcription. These results imply that relief from repression at class II promoters is a general feature of gene activation in vivo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.