ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Dynamic microtubule ends are required for growth cone turning to avoid an inhibitory guidance cue.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
66 citing papers in PubMed, 173 citations in OpenAlex.
- Analysis of PTEN Antagonistic Peptides (PAPs) in Neuronal Growth and Traumatic Brain Injury (TBI).Biomacromolecules · 2026Article
- APLP1 Interacts with SARM1 and Regulates Axonal Maintenance and Post-Injury Degeneration.Molecular neurobiology · 2025Article
- The TOG5 domain of CKAP5 is required to interact with F-actin and promote microtubule advancement in neurons.Molecular biology of the cell · 2024Article
- CKAP5 enables formation of persistent actin bundles templated by dynamically instable microtubules.Current biology : CB · 2024Article
- Tubulin post-translational modifications in meiosis.Seminars in cell & developmental biology · 2023Review
- Cellular cartography: Towards an atlas of the neuronal microtubule cytoskeleton.Frontiers in cell and developmental biology · 2023Review
- With the Permission of Microtubules: An Updated Overview on Microtubule Function During Axon Pathfinding.Frontiers in molecular neuroscience · 2021Review
- Chronic neuronal activation increases dynamic microtubules to enhance functional axon regeneration after dorsal root crush injury.Nature communications · 2020Article
- Investigating the impact of the phosphorylation status of tyrosine residues within the TACC domain of TACC3 on microtubule behavior during axon growth and guidance.Cytoskeleton (Hoboken, N.J.) · 2020Article
- XMAP215 promotes microtubule-F-actin interactions to regulate growth cone microtubules during axon guidance inJournal of cell science · 2019Article
- Knockdown of Fidgetin Improves Regeneration of Injured Axons by a Microtubule-Based Mechanism.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Article
- Human TUBB3 Mutations Disrupt Netrin Attractive Signaling.Neuroscience · 2018Article
- Model for Coordination of Microtubule and Actin Dynamics in Growth Cone Turning.Frontiers in cellular neuroscience · 2018Article
- Cue-Polarized Transport of β-actin mRNA Depends on 3'UTR and Microtubules in Live Growth Cones.Frontiers in cellular neuroscience · 2018Article
- Neuronal Growth Cone Size-Dependent and -Independent Parameters of Microtubule Polymerization.Frontiers in cellular neuroscience · 2018Article
- Nanoparticle Delivery of Fidgetin siRNA as a Microtubule-based Therapy to Augment Nerve Regeneration.Scientific reports · 2017Article
- Uncoupling of UNC5C with Polymerized TUBB3 in Microtubules Mediates Netrin-1 Repulsion.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- The microtubule plus-end-tracking protein TACC3 promotes persistent axon outgrowth and mediates responses to axon guidance signals during development.Neural development · 2017Article
- Stability properties of neuronal microtubules.Cytoskeleton (Hoboken, N.J.) · 2016Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Growth cone turning is an important mechanism for changing the direction of neurite elongation during development of the nervous system. Our previous study indicated that actin filament bundles at the leading margin direct the distal microtubular cytoskeleton as growth cones turn to avoid substratum-bound chondroitin sulfate proteoglycan. Here, we investigated the role of microtubule dynamics in growth cone turning by using low doses of vinblastine and taxol, treatments that reduce dynamic growth and shrinkage of microtubule ends. We used time-lapse phase-contrast videomicroscopy to observe embryonic chick dorsal root ganglion neuronal growth cones as they encountered a border between fibronectin and chondroitin sulfate proteoglycan in the presence and absence of 4 nM vinblastine or 7 nM taxol. Growth cones were fixed and immunocytochemically labeled to identify actin filaments and microtubules containing tyrosinated and detyrosinated alpha-tubulin. Our results show that after contact with substratum-bound chondroitin sulfate proteoglycan, vinblastine- and taxol-treated growth cones did not turn, as did controls; instead, they stopped or sidestepped. Even before drug-treated growth cones contacted a chondroitin sulfate proteoglycan border, they were narrower than controls, and the distal tyrosinated microtubules were less splayed and were closer to the leading edges of the growth cones. We conclude that the splayed dynamic distal ends of microtubules play a key role in the actin filament-mediated steering of growth cone microtubules to produce growth cone turning.
Indexed as
Identifiers
9096143PMC6573642W1614502504What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.