ArticleGenetics1997
Circularized Ac/Ds transposons: formation, structure and fate.
Article in Genetics, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Abundance of Transgene Transcript Variants Associated with Somatically Active TransgenicInternational journal of molecular sciences · 2023Article
- Isolation and Characterization ofFrontiers in plant science · 2018Article
- TED, an autonomous and rare maize transposon of the mutator superfamily with a high gametophytic excision frequency.The Plant cell · 2013Article
- A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Genome rearrangements in maize induced by alternative transposition of reversed ac/ds termini.Genetics · 2011Article
- Fusion of reverse-oriented Ds termini following abortive transposition in Arabidopsis: implications for the mechanism of Ac/Ds transposition.Plant cell reports · 2010Article
- Transpositionally active episomal hAT elements.BMC molecular biology · 2009Article
- Genome-wide analysis of Tol2 transposon reintegration in zebrafish.BMC genomics · 2009Article
- Reversed end Ds element: a novel tool for chromosome engineering in Arabidopsis.Plant molecular biology · 2008Article
- Assembly of the mariner Mos1 synaptic complex.Molecular and cellular biology · 2005Article
- Characterization of an unusual Ds transposable element in Arabidopsis thaliana: insertion of an abortive circular transposition intermediate.Plant molecular biology · 2004Article
- Transposition of reversed Ac element ends generates chromosome rearrangements in maize.Genetics · 2004Article
- Nonhomologous-end-joining factors regulate DNA repair fidelity during Sleeping Beauty element transposition in mammalian cells.Molecular and cellular biology · 2003Article
- FARE, a new family of foldback transposons in Arabidopsis.Genetics · 2000Article
- Analysis of extrachromosomal Ac/Ds transposable elements.Genetics · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The maize Ac/Ds transposable elements are thought to transpose via a cut-and-paste mechanism, but the intermediates formed during transposition are still unknown. In this work we present evidence that circular Ac molecules are formed in plants containing actively transposing elements. In these circles, transposon ends are joined head-to-head. The sequence at the ends' junction is variable, containing small deletions or insertions. Circles containing deleted Ac ends are probably unable to successfully reintegrate. To test the ability of circles with intact transposon ends to integrate into the genome, an artificial Ds circle was constructed by cloning the joined ends of Ac into a plasmid carrying a plant selectable marker. When such a circular Ds was introduced into tobacco protoplasts in the presence of Ac-transposase, no efficient transposase-mediated integration was observed. Although a circular transposition intermediate cannot be ruled out, the findings of circles with deleted transposon ends and the absence of transposase-mediated integration of the circular Ds suggest that some of the joined-ends-carrying elements are not transposition intermediates, but rather abortive excision products. The formation of Ac circles might account for the previously described phenomenon of Ac-loss. The origin of Ac circles and the implications for models of Ac transposition are discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.