ArticleNucleic acids research1997
Profile of the DNA recognition site of the archaeal homing endonuclease I-DmoI.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- F-CphI represents a new homing endonuclease family using the Endo VII catalytic motif.Mobile DNA · 2018Article
- A group II intron encodes a functional LAGLIDADG homing endonuclease and self-splices under moderate temperature and ionic conditions.RNA (New York, N.Y.) · 2010Article
- Homing endonucleases: from basics to therapeutic applications.Cellular and molecular life sciences : CMLS · 2010Review
- Phylogenetic screening of a bacterial, metagenomic library using homing endonuclease restriction and marker insertion.Nucleic acids research · 2009Article
- High-resolution profiling of homing endonuclease binding and catalytic specificity using yeast surface display.Nucleic acids research · 2009Article
- Generation of a nicking enzyme that stimulates site-specific gene conversion from the I-AniI LAGLIDADG homing endonuclease.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Optimization of in vivo activity of a bifunctional homing endonuclease and maturase reverses evolutionary degradation.Nucleic acids research · 2009Article
- Crystal structure of I-DmoI in complex with its target DNA provides new insights into meganuclease engineering.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Coevolution of a homing endonuclease and its host target sequence.Journal of molecular biology · 2007Article
- Evolution of Pleopsidium (lichenized Ascomycota) S943 group I introns and the phylogeography of an intron-encoded putative homing endonuclease.Journal of molecular evolution · 2007Article
- Degenerated recognition property of a mitochondrial homing enzyme in the unicellular green alga Chlamydomonas smithii.Plant molecular biology · 2006Article
- Analysis of the LAGLIDADG interface of the monomeric homing endonuclease I-DmoI.Nucleic acids research · 2004Article
- Degeneration of a homing endonuclease and its target sequence in a wild yeast strain.Nucleic acids research · 2001Article
- Rapid evolution of the DNA-binding site in LAGLIDADG homing endonucleases.Nucleic acids research · 2001Article
- Functional alpha-fragment of beta-galactosidase can be expressed from the mobile group I intron PpLSU3 embedded in yeast pre-ribosomal RNA derived from the chromosomal rDNA locus.Nucleic acids research · 2000Article
- Genetic analysis of the Chlamydomonas reinhardtii I-CreI mobile intron homing system in Escherichia coli.Genetics · 1997Article
- Statistical modeling and analysis of the LAGLIDADG family of site-specific endonucleases and identification of an intein that encodes a site-specific endonuclease of the HNH family.Nucleic acids research · 1997Article
- Purification, biochemical characterization and protein-DNA interactions of the I-CreI endonuclease produced in Escherichia coli.Nucleic acids research · 1997Article
- Mapping metal ions at the catalytic centres of two intron-encoded endonucleases.The EMBO journal · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
I- Dmo I is a homing enzyme of the LAGLI-DADG type that recognizes up to 20 bp of DNA and is encoded by an archaeal intron of the hyperthermophilic archaeon Desulfurococcus mobilis . A combined mutational and DNA footprinting approach was employed to investigate the specificity of the I- Dmo I-substrate interaction. The results indicate that the enzyme binds primarily to short base paired regions that border the sites of DNA cleavage and intron insertion. The minimal substrate spans no more than 15 bp and while sequence degeneracy is tolerated in the DNA binding regions, the sequence and size of the cleavage region is highly conserved. The enzyme has a slow turnover rate and cuts the coding strand with a slight preference over the non-coding strand. Complex formation produces some distortion of the DNA double helix within the cleavage region. The data are compatible with the two DNA-binding domains of I- Dmo I bridging the minor groove, where cleavage occurs, and interacting within the major groove on either side, thereby stabilizing a distorted DNA double helix. This may provide a general mode of DNA interaction at least for the LAGLIDADG-type homing enzymes.
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