ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Identification of the single channels that underlie the N-type and L-type calcium currents in bullfrog sympathetic neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Single channel measurements demonstrate the voltage dependence of permeation through N-type and L-type CaV channels.Channels (Austin, Tex.) · 2015Article
- Voltage control of Ca²⁺ permeation through N-type calcium (Ca(V)2.2) channels.The Journal of general physiology · 2014Article
- Identification of CaV channel types expressed in muscle afferent neurons.Journal of neurophysiology · 2013Article
- Domain III regulates N-type (CaV2.2) calcium channel closing kinetics.Journal of neurophysiology · 2012Article
- (R)-roscovitine prolongs the mean open time of unitary N-type calcium channel currents.Neuroscience · 2010Article
- Alternative splicing in the C-terminus of CaV2.2 controls expression and gating of N-type calcium channels.The Journal of physiology · 2006Article
- Neuronal L-type calcium channels open quickly and are inhibited slowly.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Electrophysiological properties of BK channels in Xenopus motor nerve terminals.The Journal of physiology · 2004Article
- Elementary properties of axonal calcium currents in type B photoreceptors in Hermissenda crassicornis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- cGMP/protein kinase G-dependent inhibition of N-type Ca2+ channels induced by nitric oxide in human neuroblastoma IMR32 cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Direct measurement of single-channel Ca(2+) currents in bullfrog hair cells reveals two distinct channel subtypes.The Journal of physiology · 2001Article
- G-protein inhibition of N- and P/Q-type calcium channels: distinctive elementary mechanisms and their functional impact.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Low-affinity blockade of neuronal N-type Ca channels by the spider toxin omega-agatoxin-IVA.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Reluctant gating of single N-type calcium channels during neurotransmitter-induced inhibition in bullfrog sympathetic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Auxiliary subunits operate as a molecular switch in determining gating behaviour of the unitary N-type Ca2+ channel current in Xenopus oocytes.The Journal of physiology · 1999Article
- Gating of single N-type calcium channels recorded from bullfrog sympathetic neurons.The Journal of general physiology · 1999Article
- Overview of voltage-dependent calcium channels.Journal of bioenergetics and biomembranes · 1998Review
- Mechanisms of modulation of voltage-dependent calcium channels by G proteins.The Journal of physiology · 1998Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Most of the whole-cell calcium current of frog sympathetic neurons is an N-type current, blocked by omega-conotoxin GVIA (omegaCGVIA). Thus, these cells should be an excellent system to study the properties of single N-type channels. However, a channel that is active near -10 mV in isotonic Ba2+, originally identified as "N-type," corresponds more closely to a omegaCGVIA-resistant component of the whole-cell current observed in 100 mM Ba2+. That conclusion would imply that the true single-channel correlate of the macroscopic N-current remains to be identified in frog sympathetic neurons. I report here recordings from cell-attached patches of a calcium channel that activates in the appropriate voltage range (>0 mV, in isotonic Ba2+) and is blocked by omegaCGVIA. This channel has a slope conductance of 20 pS (range, 17-25 pS) and a single-channel current of -1.3 pA at 0 mV. Other channels active in the same voltage range (24 pS, -1.3 pA at 0 mV) were identified as L-type channels because they exhibited long openings after repolarization in the presence of 1 microM Bay K 8644 and were resistant to omegaCGVIA. A third channel type (13-19 pS) was distinguished by current amplitude (-0.6 pA at 0 mV) and strong inactivation at -40 mV. The similarity in slope conductance among these channels demonstrates that distinguishing them requires the consideration of additional properties. The omegaCGVIA-sensitive channel can be identified as an N-type calcium channel.
Indexed as
Identifiers
9092587PMC6573110W1674110592What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.