ArticleThe Journal of experimental medicine1997
T cell receptor (TCR) mini-gene mRNA expression regulated by nonsense codons: a nuclear-associated translation-like mechanism.
Article in The Journal of experimental medicine, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 77 citations in OpenAlex.
- Stress and the nonsense-mediated RNA decay pathway.Cellular and molecular life sciences : CMLS · 2017Review
- 5-azacytidine inhibits nonsense-mediated decay in a MYC-dependent fashion.EMBO molecular medicine · 2014Article
- Unspliced precursors of NMD-sensitive β-globin transcripts exhibit decreased steady-state levels in erythroid cells.PloS one · 2012Article
- Perturbation of thymocyte development in nonsense-mediated decay (NMD)-deficient mice.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Frame-disrupting mutations elicit pre-mRNA accumulation independently of frame disruption.Nucleic acids research · 2010Article
- Respiratory syncytial virus infection alters surfactant protein A expression in human pulmonary epithelial cells by reducing translation efficiency.American journal of physiology. Lung cellular and molecular physiology · 2009Article
- Nonsense codons trigger an RNA partitioning shift.The Journal of biological chemistry · 2009Article
- Unexpected roles for UPF1 in HIV-1 RNA metabolism and translation.RNA (New York, N.Y.) · 2008Article
- Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay.The Journal of clinical investigation · 2008Article
- Inhibition of the ubiquitin-proteasome system induces stress granule formation.Molecular biology of the cell · 2007Article
- An alternative branch of the nonsense-mediated decay pathway.The EMBO journal · 2007Article
- AUG sequences are required to sustain nonsense-codon-mediated suppression of splicing.Nucleic acids research · 2006Article
- Nonsense-associated alternative splicing of T-cell receptor beta genes: no evidence for frame dependence.RNA (New York, N.Y.) · 2005Article
- Stop codon-mediated suppression of splicing is a novel nuclear scanning mechanism not affected by elements of protein synthesis and NMD.RNA (New York, N.Y.) · 2004Article
- Unspliced Rous sarcoma virus genomic RNAs are translated and subjected to nonsense-mediated mRNA decay before packaging.Journal of virology · 2004Article
- Premature termination codons do not affect the rate of splicing of neighboring introns.RNA (New York, N.Y.) · 2004Article
- A nuclear translation-like factor eIF4AIII is recruited to the mRNA during splicing and functions in nonsense-mediated decay.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Preferential expression of hPGFS in primary SCCHN and tumour cell lines derived from respiratory and digestive organs.British journal of cancer · 2004Article
- A Hox gene mutation that triggers nonsense-mediated RNA decay and affects alternative splicing during Drosophila development.Nucleic acids research · 2003Article
- A quantitative analysis of intron effects on mammalian gene expression.RNA (New York, N.Y.) · 2003Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Premature termination codons (PTCs) are known to decrease mRNA levels. Here, we report our investigation of the mechanism for this downregulation using the TCR-beta gene, which acquires PTCs as a result of programmed rearrangements that occur during normal thymic development. We found that a mini-gene version of this gene, which contains only three TCR-beta exons, exhibited efficient downregulation in response to PTCs. This demonstrates that the full coding sequence is not necessary for appropriate regulation. Mutation of the translation start AUG and a downstream in-frame AUG that displayed similarity to the Kozak consensus sequence reversed the downregulatory response to PTCs. Thus, an AUG start codon is required to define the reading frame of a PTC. Specific suppressor tRNAs also reversed the downregulatory response, strongly implicating the involvement of a translation-like process. Remarkably, the addition of suppressor tRNAs or the inactivation of the start AUGs caused a dramatic rise in the levels of PTC-bearing transcripts in the nuclear fraction prepared by two independent methods. Collectively, our results provide evidence for a codon-based surveillance mechanism associated with the nucleus that downregulates aberrant transcripts encoding potentially toxic polypeptides from nonproductively rearranged genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.